We narrowed to 129 results for: primer

...features to help with the post-design process such as primer and plasmid design. Examples:  “multiplex design...or multiplex features. However, tools aiding in primer design are more and more common. User interaction...

...Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT...10 μL 1,000 μL 100 μM Forward Primer 0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water...GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free... SYBR master mix, then the forward and reverse primers. Vortex briefly, immediately before use. Use a ... of ~70–75 °C usually indicates the presence of primer dimers which can increase background signal and...

...on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors ...

...amenability to genetic manipulation.  This is just a primer so that you are aware of at least some of the possibilities...

...chatting quickly with the postdoc who’s really good at primer design – but first, you have to know what resources...

...amplified using the same sets of forward and reverse primers). The abundance of a particular barcode sequence...

...or toe clip) and using PCR with the appropriate primers to detect your targeting vector or gene of interest...

... Addgene Blog New to Virus Work? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about...

...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

...stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into some of the important...