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We narrowed to 541 results for: SHI

Showing: 161 - 180 of 541 results

  1. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...communication. When I started my Science Communication Internship with Addgene, I didn’t know a lot about scicomm... few Addgene guest blog pieces. Throughout my internship, my interest in scicomm has grown and now it ...is Hans Packer - science writer  When Hans was finishing up his PhD in Molecular Neuroscience at the University...Technologies (IDT). “It was fairly accidental. I was finishing graduate school, and didn’t have anything lined...

  2. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...to sign up for a writing intensive weekend in Washington D.C. at the ASM headquarters. I decided to go...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

  4. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a .... They’re really easy to spot and even easier to ship back. Be that person! If you use serological pipettes...in your tissue culture room for the wrappers and ship them back. Start a glove recycling program at ...

  5. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ...Waardenburg Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol University’s Ramathibodi Hospital... Addgene will make a substantial difference in pushing the work forward. Xeroderma Pigmentosum Hendrik...precisely.  To put this award into perspective, Shiao wrote in an email: “In Thailand, our annual operating...

  6. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...infrastructure and offered to collaborate with them. The partnership was fruitful and a first version of the study... the power of collaboration and join efforts to shift scientific culture towards a more generous and rewarding...asymmetric collaborations.” These are transient partnerships where a researcher makes a small but crucial...from becoming starting points for long-lasting partnerships Join us! We are a community of researchers who...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...which can help determine structure and function relationships or, in more practical applications, allow a ...some systems, rare codons and four-amino acid frameshift codons are also used. Note that some systems ...idiosyncrasies presents a significant barrier for those wishing to use it as a tool. In short, while the technical... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9... to control transcription. Two separate labs, Moritoshi Sato’s lab at the University of Tokyo and Charles... site of AcrIIA4, this unfolding conformational shift can interfere with activity. The resulting system...Niopek D (2020) Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology...Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable...

  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...is the question. When presenting on building relationships (also known as “networking”), one of the most...networking to start, build, and track professional relationships. Here are my seven best tips beyond the basics...on LinkedIn!” resulting in just a little more relationship. Pro tip: Once you are connected with someone...connection requests LinkedIn is a place to build relationships. If you don’t add a personal note, you waste...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ...teachers; organizing Congressional briefings in Washington DC; convening meetings of experts and leaders...the biological sciences, as well as to acquire leadership and communication experience. These included ... lectures, writing science articles for a lay readership, and working with middle and high school students...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...

  11. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing...light-absorbing chromoprotein that Hideji Murakoshi’s lab developed as an acceptor for fluorescence lifetime...https://doi.org/10.1074/jbc.m606921200 Dove SG, Takabayashi M, Hoegh-Guldberg O (1995) Isolation and Partial...1). https://doi.org/10.1186/s13036-018-0100-0 Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato,...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...properties once exclusive to small-molecules: red-shifted spectra, ion sensitivity, photoactivation, etc....sample is wholly dependent on the photon budget and pushing the frontiers of fluorescence microscopy often ...can feel like a college student searching couch cushions for spare change, desperate to extract a few more...which is particularly important for samples where washing is difficult, such as intact tissue. Some chemical...

  14. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...insertion that disrupts fluorescence due to a frameshift. Restoration of fluorescence can only occur through...Harris Lab relies on the restoration of a frame-shift in mCherry that restores fluorescence to monitor...tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus a successful base editing...with a cytidine deaminase base editor results in shift in emission spectra from BFP to GFP. Image from ...

  15. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...two independent groups (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering...27096365. PubMed Central PMCID: PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using...25733849. PubMed Central PMCID: PMC4371917. 5. Shimatani, Zenpei, et al. "Targeted base editing in rice...PubMed Central PMCID: PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing...

  16. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...for. Secondly, categorize your edit – is it any frameshift mutation? A specific insertion or deletion? A...line is desired, any mutation that introduces a frameshift in the coding region of interest will get the...and run the PCR product on a gel to look for size shifts. Once you’ve identified homozygous clones, however...large so that you can easily visualize the size shift you anticipate. A good rule of thumb is to keep ...

  17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...initiation heavily dependent upon the core of a Shine-Delgarno sequence found at the 3’ end of the leader...was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called bicistronic...Academy of Sciences 113.9 (2016): 2388-2393. Huang, Shijie, Xuechen Zhu, and Charles E. Melançon III. "Detection...ChemBioChem 17.10 (2016): 895-899. Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized...

  18. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...own piece (3). After incubation, blocking, and washing, the membrane is reassembled and imaged. In this...(PBS) are the most commonly used solutions for washing. If your background noise is high, you can consider...Like blocking, you don’t want to skimp on your washing steps! It’s important to let them wash for the ...sequence not accurate Check plasmid sequence for frameshifts and stop codons Weird background patterns...primary antibody at 4 °C overnight Incomplete washing Ensuring wash is happening under sufficient agitation...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...efficiency and specificity. With TurboCas, the Ali Shilatifard Lab was able to identify both new and known regulators...miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created a very quick and efficient way to...Morgan, M. A., Andersen, G. D., Bartom, E. T., & Shilatifard, A. (2024). TurboCas: A method for locus-specific....2024.11.007. Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. ...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...blue-shifted fluorescent protein (donor) and measuring the resultant emission from the red-shifted fluorescent...imaging. The solution is to couple Nluc with a red-shifted fluor. Here, NLuc internally excites the fluor ...these tools, NanoLuc-fluorescent protein fusion shifts NLuc’s bright blue luminescence to wavelenghts ...
Showing: 161 - 180 of 541 results