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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 313 results for: bon;

Showing: 241 - 270 of 313 results

  2. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...experiments.       References and resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence...

  3. Typing CRISPR Systems

    Type
    Blog Post
    ...Laderman, E., Armbrust, M., Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning...

  5. Biosensor AAV Preps

    Type
    Collection
    ...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51083 AAV-EF1a-DIO-GCaMP6f-...GSG-P2A-GCaMP6f-WPRE-pA CAG GCaMP6f mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Bonhoeffer , Huebener , Rose 51082 AAV-EF1a-DIO-GCaMP6s-...GSG-P2A-GCaMP6s-WPRE-pA CAG GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 68720 pAAV-hSyn1-Flex-mRuby2...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Cre dependent 1 Bonhoeffer , Huebener , Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

  10. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primers. These primers typically anneal to the backbone and can help you verify the ends of the insert...primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference...

  11. Cre-lox system

    Type
    Collection
    ... mNanog Mammalian Hanna 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase...expression EFS AAV Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA coexpression...coexpression Cbh AAV Zhang 60231 AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged...genOway press release for more information. Empty backbones to regulate your gene of interest: ID Plasmid ... Shivdasani 18925 pJ241-FLEX FLEX switch empty backbone Sternson 38042 pAAV-CA-FLEX Cre activates gene...

  12. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...

  14. Ligation Independent Cloning

    Type
    Protocol
    ...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...

  15. Zebrafish Plasmid Collection

    Type
    Collection
    ...This includes plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, ...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources...pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing...

  17. Kit Free RNA Extraction

    Type
    Protocol
    ...most mammalian tissues; more hardy tissues such as bone, or bacteria/yeast/plant samples will require additional...

  18. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...prokaryotic survival, but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR...prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 Bondy-Denomy J, Garcia B, Strum S, Du M, Rollins MF, Hidalgo-Reyes...proteins. Nature . 526(7571):136-9. PMID: 26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013...

  19. Fluorescent Protein Guide

    Type
    Collection
    ...Fluorescent Protein Plasmids by Category Empty Backbones Understand the localization and/or function of...fluorescent proteins, as well as over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based...

  20. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Imaging More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background...near-infrared , and photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in...

  21. Plasmid Collections

    Type
    Collection
    ...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi... are a set of plasmids all built with the same backbone and only differing in a small region. Click here...

  22. Mammalian RNAi Tools

    Type
    Collection
    ...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...

  23. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...reaction which ligates a promoter into an empty backbone can be repeated to ligate your gene of interest...omitted from the coding sequence of the part and backbone. For some standards, there are additional sites...

  24. Worm Expression Resources

    Type
    Collection
    ...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples...

  25. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human...RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has built a library...

  26. CRISPR References and Information

    Type
    Collection
    ...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597...

  27. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library 169942 Knockout Human Ebert 3rd 4 2,852 Bonifacino Lab Human ubiquitination-related proteins CRISPR...CRISPR KO library 174592 Knockout Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries...

  29. Plan Your Experiment

    Type
    Collection
    ...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

  30. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid Collection...this fusion protein guide to find the appropriate backbone). Then, by examining the overlap between your ...
Showing: 241 - 270 of 313 results