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Showing: 241 - 270 of 782 results

  1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...microscopy, University of Amsterdam. The previous two posts in this series described a practical approach to... a photostable fluorescent protein. In the third post of this series, we will discuss how to select a ...

  2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular... 1 of your next paper or thesis. This series of posts explains how to critically assess the reported properties... imaging that will be treated in this series of posts are (i) brightness, (ii) photostability and (iii...

  3. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...This blog post was contributed by guest bloggers Roodolph (Roo) P. St Pierre and Rose C. St Pierre, founders...scholarships, fellowships, and entry-level positions post graduation. Many thanks to our guest bloggers ...researchers Applying for jobs? Check out these blog posts  ...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt...doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair...

  6. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...be included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  8. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ...and a positive perspective on the transition to a post-academic career. This training was “included” with...Addgene's science career resources Browse all blog posts related to science careers Download the Science ... it’s largely a matter of luck if a student or postdoc ends up in a lab where they can learn the plus ...development as core components of predoctoral and postdoctoral training. A grant from the National Institute...

  9. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...focused lectures, interactive panels, and a student poster session. All DayCon lecture content is open source...

  10. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J ...Addgene's Synthetic Biology Overview More Synbio Blog Posts ...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...This post was contributed by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience...The Rockefeller University. In this and upcoming posts in this optogenetics primer series, I’m happy to...result in a particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses...expression of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression...material science part of optogenetics. In next week’s post we’ll delve into some of the important materials...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  12. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...microscopy, University of Amsterdam. The previous post in this series described a practical approach to...selecting a bright fluorescent protein. In the second post of this series, we will discuss how to select a ...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the ... from the Allen Institute for Cell Science. This post was contributed by the gene editing team at the ...Non-Homologous End Joining Check Out Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging...

  15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...advantages that will be discussed in detail in a separate post. Focusing on the ccdB aspect, Gateway takes advantage...selecting for recombinant plasmids. Check out our blog post on Common Lab E. coli strains for more strain info...Golden Gate Cloning Read all of our Plasmid Cloning Posts Resources on Addgene.org Choose a Molecular Cloning...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...where he is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor at...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...within transmembrane proteins, such as receptors, or post-translational modifications on those proteins, like...decrease in signal strength in a flow plot. d = days post treatment. Created using biorender.com. Cell...Conclusion Congratulations! You made it through this blog post and hopefully learned a few new methods of target...briefly walk through the topics we covered in this post. Besides direct labeling, indirect labeling can ...

  18. A Control for All Seasons

    Type
    Blog Post
    ...like experiments (and their controls!). This blog post will discuss the ins and outs of controls for biological...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be run...flow cytometry experiments. We hope that this blog post will be a useful starting point for you when designing...antibody-based experiments, check out these blog posts.       References and resources References Burry...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...sometimes inte-great-ion isn’t so great. In this post, we’ll cover how standard lentiviral vectors work...proteins packaged during production (read our blog post on the retroviral life cycle for a more detailed...titration of vector DNA in samples harvested 24 hours post-transduction (Wanisch and Yáñez-Muñoz, 2009).  ...insert themselves into every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References...

  20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow... utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer...View the overview schematic at the bottom of this post for a basic synopsis of the differences amongst ...due to the addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly...

  21. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected...Bacterial Expression Systems. Check out our companion post describing protein expression strains, where we ...

  22. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...viral service page and browse our viral vector blog posts for many more tips and ideas on how to use these...Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources on Addgene.org Check out ...

  23. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...protocols for different experiments.  In this blog post let's take a look at some of the other open resources...   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study...Cas9 in C. elegans Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids...

  24. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ... sure you’re familiar with the policies before posting something on social media. If someone is presenting...and Meet People at a Scientific Conference” blog post. There’s so many great tips for getting the most...conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific communication,...you have a poster or talk at the conference, you can end by asking them to come to your poster or talk. ...be beneficial. The poster session Before the conference If you’re presenting a poster at a conference, ...people will visit your poster: those that want you to walk them through the entire poster, or those that want... the poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session...

  25. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... find themselves faced with a roadblock. In this post, we will provide an overview of some of the common... tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing... to check out the lentiviral protocols and blog posts on Addgene’s website for additional resources. ...

  26. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit spreadsheet...“How to Deposit Your Plasmids with Addgene” blog post for a thorough guide to our online data entry process...discoveries with scientists around the world. This post was originally written by Tyler Ford in March 2017...

  27. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation...ultimately gene-expression. Polyadenylation is the post-transcriptional additional of multiple adenine (...Cloning Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference...Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of...

  28. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience...excitation spectrum (use the resources discussed in post one of this series already provided or check the... to the fun stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  29. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our ...career articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

  30. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...Biophysics from UC Davis in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University...Terriers. More SynBio Reading Read more Addgene blog posts about synthetic biology ...was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had started...
Showing: 241 - 270 of 782 results