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We narrowed to 569 results for: CIT

Showing: 161 - 180 of 569 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... abandoned early on due to its considerable immunogenicity and its transient expression. LV results in...integration into the genome, and its large DNA packaging capacity is well suited for larger transgenes. Unfortunately...to cause any disease in humans, and its low immunogenicity is critical when systemic delivery of high ...and safer. It is now one of the most active and exciting areas of science. This is hopefully only the beginning...

  2. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...scientists enjoy is discussion of current new and exciting literature. As I transitioned out of academia ...opportunity to continue such discussions. Beyond the excitement of learning about new cutting edge science, researchers...first meeting, now what? After the first meeting solicit feedback. To ensure that the journal club is useful...accommodates more people. The feedback could be solicited via multiple avenues including casual request ...

  3. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...vector performance) will be assigned citable DOIs through DataCite before being made publicly available...experiments. By providing DOIs, this information can be cited, tracked, and appropriately credited, broadening...lines of “we have a lot”. In fact, scientists often cite “word of mouth” as their source for this type of...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing... may have lower signal, but they have higher specificity, lower cross-reactivity, and are more consistent... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...Increased time; have to make as-needed Increase specificity Monoclonal antibodies Increased cost ...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described recently...yield the same BDNF protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab...reprogramming, and disease modeling. Further, the paucity of Cre-dependent mouse lines for overexpressing...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... for in vivo gene delivery due to their low immunogenicity and range of serotypes allowing preferential...AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has been...separate AAV vectors, increasing overall packaging capacity but necessitating purification and co-infection...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...autofluorescence The cyan light that is used to excite GFP, may also excite several components that are naturally...autofluorescence is less of a problem when the excitation wavelength is shifted to longer wavelengths. ...chemical reaction and hence, there is no need to excite the sample. Although bioluminescent probes are ...

  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...two HIV-1 infected patients. Although this is an exciting finding, it’s important to note that these HIV... HIV-1 therapy Both of these methods represent exciting advances in preclinical HIV-1 research that should...challenge for CRISPR HIV-1 therapies is sequence specificity. Whereas antiviral therapies target HIV-1 at ...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...features and limitations of existing backbones, we're excited to share our very own set of empty, HA-, and EGFP-expressing...TurboCas improves on these in both efficiency and specificity. With TurboCas, the Ali Shilatifard Lab was able...characterized these tools and validated their specificity in mice, non-human primates, and human tissue...layers (L1-6) and scale bar are indicated. B) Specificity, as percentage of BiPVe3-dTomato and PV-positive...

  10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...type depends largely on the viral capsid, but specificity doesn’t end there: expression from rAAVs also...for mice in vivo. To target... Try using... Citation CNS AAV1, AAV2, AAV5, AAV8, AAV9, AAVrg Aschauer...Medicine, 5(189), 189ra76. https://doi.org/10.1126/scitranslmed.3005708 Goertsen, D., Goeden, N., Flytzanis,...Transduction but Differ in Sialic Acid Linkage Specificity. Journal of Virology, 75(15), 6884–6893. https...

  11. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...Messing, A., & Brenner, M. (2004). Expression specificity of GFAP transgenes. Neurochemical Research, 29...MicroRNA124 Target Sequence Restores Astrocyte Specificity of gfaABC1D-Driven Transgene Expression in AAV-Mediated...

  12. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...itself, please read on to learn more about this exciting technology from the folks at Transposagen. This...Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated) with no known...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...

  13. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...possible to control neuronal activity with a specificity far greater than the one that can be achieved...channelrhodopsins that, in turn, trigger neural excitation, whereas yellow light activates halorhodopsins...Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...Zoé Christenson, and Esther Krook-Magnuson. "Specificity, versatility, and continual development: the ...

  14. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...important to note that naked and unmodified dsRNA elicits immunogenic reactions that complicate the effectiveness...the most prevalent method due to ease of use and capacity to generate a long-term RNAi phenotype. Long hairpin...transitivity,” calls into question any claims about specificity of a given RNAi technique. While transitivity...Steinmetz LM, Drewes G, Huber W (2019) Biological plasticity rescues target activity in CRISPR knock outs....

  15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...stimulate or inhibit neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other...limited to only a few kb so attaining neuronal specificity through promoter-directed expression alone is...generic promoter). A DIO opsin allows cell type specificity dependent on the promoter of the cre recombinase...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...

  16. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ... kit pages as well! Some kits that may not be explicitly labeled as “yeast”, “transfection-grade”, or ...

  17. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ...it be nice if those weeks and months of work was cited by others?)  Ready to submit?  Fantastic! The next...

  18. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...hospitalization for gynaecologic disorders” in Ghana. We’re excited to see what impact Dr. Bengyella’s work will have... chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass...Seeding Labs’ Instrumental Access program. We are excited to discover what new and creative ideas applicants...

  19. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...scientists using Addgene in their labs. And it has been exciting to learn about all the amazing research being ...neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT...community. The scientists we've spoken with are excited and passionate about making such tools open-access...

  20. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...plasmids and 6,000 biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch...importantly, what doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used...its ever-evolving needs. For now, we have many exciting things planned in 2023, from the expansion of ...
Showing: 161 - 180 of 569 results