We narrowed to 191 results for: CO

1. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...killer”) cells. Typically, you would label the CD8 co-receptor protein on the surface of the CD8+ T cells...

2. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   ... (ITAM). By fusing ITAM to an RTK of interest and co-expressing a cytosolic ZtSH2 reporter, they observe...

3. Using Phosphoserine to Study Protein Phosphorylation

   Type

   Blog Post

   ...type’ control can be made one of two ways. First, co-expression of a suppressor tRNA (SupD) instead of...

4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   ...each due to size limitations associated with AAV; co-infection with both constructs produces a minimum...

5. Colony Formation Titering Assay

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   Protocol

   ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

6. Technologies Enabled by NanoLuc® Luciferase

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   Blog Post

   ...design a better fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation...

7. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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   Blog Post

   ...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma...

8. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...targeted cytosine methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting ...

9. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   ...interesting protein targets and you want to know if they co-localize at the same place in the cell? Or you know...

10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type

    Blog Post

    ...below). In the absence of extra recombinase-specific co-factors (which should be excluded from the system...

11. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

12. Gamma-Retroviral Vector Guide

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    Guide

    ...described above (envelope, packaging, and transfer) are co-transfected into the HEK293T packaging cell line....

13. Transferable Skills Guide: Teamwork

    Type

    Blog Post

    ...joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally...

14. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp. Front Cell...

15. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    ...serotypes utilize a number of different receptors, co-receptors, and other mechanisms to gain entry into...

16. Luciferase Plasmid Collection

    Type

    Collection

    ...upstream of Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB...

17. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    ...their cell-surface proteome to inhibit expression of co-stimulatory molecules needed for T cell effector ...

18. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    ... two equally sized digestion products (that would co-migrate on a gel) instead of two different sized ...

19. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post

    ...dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta...

20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    ...good option. Originally developed by Roger Tsien and co-workers (Griffin, Adams, & Tsien, 1998), these dyes...