Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 438 results for: ERG

Showing: 161 - 180 of 438 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

  2. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

  5. Zebrafish Plasmid Collection

    Type
    Collection
    ...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    .... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...

  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go against...this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is done...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...varieties must stay current with literature and emerging technologies to remain competitive and relevant...scientists that aims to help stay current on new and emerging work by collaboratively discussing recent publications...

  14. MXS Chaining

    Type
    Blog Post
    ... Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid... PMCID: PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid...

  15. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...https://doi.org/10.1126/science.1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung....2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal...PMC4028491. Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...positioned PAM without undesired bases nearby that may undergo unwanted editing. Just like with base editing, ... and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally the strongest...

  19. Viral Production at Addgene

    Type
    Blog Post
    ...pool. In addition, the final viral preparation undergoes PCR with primers targeting the transfer plasmid...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...symptoms of the disease. However, there are some emerging treatments that have shown some positive potential...as these new detection techniques and therapies emerge into mainstream use, they will have a much greater...
Showing: 161 - 180 of 438 results