We narrowed to 435 results for: ERG

... by ion channels and pumps in the membrane. An emerging interdisciplinary field of biology known as bioelectricity...Developmental bioelectricity is an extremely exciting emerging field, with many implications for regenerative...

...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

...experiments that requires modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses... J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale...

...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

...biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring two FPs on a ...the ability of one FP to nonradiatively transfer energy to the second FP, often decreasing the fluorescent...

...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

...fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer from one FP (e.g. CFP...

...during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go against...this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is done...

...Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring...

.... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...

...varieties must stay current with literature and emerging technologies to remain competitive and relevant...scientists that aims to help stay current on new and emerging work by collaboratively discussing recent publications...

... Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid... PMCID: PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid...

...vector allows you to visualize which cells have undergone recombination. Reporter plasmids can also be useful...

...at the German Cancer Research Center (DKFZ) in Heidelberg. CRISPR FAQ forum (Link opens in a new window...

...positioned PAM without undesired bases nearby that may undergo unwanted editing. Just like with base editing, ... and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally the strongest...

...pool. In addition, the final viral preparation undergoes PCR with primers targeting the transfer plasmid...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

...https://doi.org/10.1126/science.1231143  Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung....2014.02.001  Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova...

...symptoms of the disease. However, there are some emerging treatments that have shown some positive potential...as these new detection techniques and therapies emerge into mainstream use, they will have a much greater...