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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 430 results for: ERG

Showing: 161 - 180 of 430 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its serum, ...https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for the visualization...14166766. PubMed Central PMCID: PMC300121. 3. Söderberg, Ola, et al. "Direct observation of individual...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

  7. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

  8. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

  10. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...during exocytotic budding, as the viral particle emerges. Retrovirus life cycle Retroviruses go against...this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is done...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...varieties must stay current with literature and emerging technologies to remain competitive and relevant...scientists that aims to help stay current on new and emerging work by collaboratively discussing recent publications...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    .... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...

  18. MXS Chaining

    Type
    Blog Post
    ... Pubmed PMID: 19363495. 4. Josse J, Kaiser AD, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid... PMCID: PMC4409215. 7. Swartz MN, Trautner TA, Kornberg A. Enzymatic synthesis of deoxyribonucleic acid...

  20. Viral Production at Addgene

    Type
    Blog Post
    ...pool. In addition, the final viral preparation undergoes PCR with primers targeting the transfer plasmid...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...
Showing: 161 - 180 of 430 results