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Showing: 161 - 180 of 300 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule tracking... T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic granules ...

  3. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...

  5. TALEN Expression Vectors

    Type
    Collection
    ...have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The expression vectors...

  6. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  7. Institute for Protein Innovation

    Type
    Collection
    ...conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection includes...

  11. Retrovirus Plasmids

    Type
    Collection
    ...bicistronic IRES-mCherry containing retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... human codons, additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease ...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10...

  14. Microbiology Resources

    Type
    Collection
    ...from networks and gene regulation to sensing and signaling, to perform your synbio experiment. Synthetic ...

  15. Arf GTPase Family

    Type
    Collection
    ...triad, with each component involved in GTPase signaling activity. Figure 1: Locations of ARF superfamily...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...zebrafish astrocytes. Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins...
Showing: 161 - 180 of 300 results