We narrowed to 875 results for: LIC

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...Institute for Cell Science has produced the first publicly available collection of fluorescently tagged, ...

... tools for familiar applications. But how does one invent a tool for applications that don’t even exist... Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that ...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...reproduced from Frank Hayward et al. 2024 under CC-BY license. They did more experiments to see if any small...reproduced from Frank Hayward et al. 2024 under CC-BY license.   Video 1: Fluorescence movie of E. coli...

...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic...labeled through click chemistry. Although these multi-component systems can be complicated to implement,...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...adapted from (Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling tags on...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...reused from (Ulrichs et al., 2023) under CC-BY license. But these tags aren’t limited to fluorescent...

... you'll need Sanger sequencing reads from PCR amplicons that cover your locus of interest and correspond...intuitive interpretation of results produced by amplicon sequencing. The updated CRISPresso2 can analyze...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...

... ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication. ITRs are...binding site for Rep78 and Rep68 to initiate replication and a terminal resolution site that serves as...are thought to be self-repaired during the AAV replication process (Chen et al., 2004). Mutations in the... Rep proteins are important for viral genome replication, these mutations can affect transduction efficiency... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and... and hairpin structures, ITRs are prone to replication errors and disruptive mutations and truncations...

...because of its relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside.... Reproduced from Wu et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ... Confocal image of CA2 neurons in a mouse brain slice expressing PinkyCaMP; scale bar 50 µm. Reproduced...from Fink & Imai et al. (2024) under CC BY-NC-ND license. B) Confocal images showing fore-, mid-, and hindbrain...Zhou, Zhu, Eom, Fang, et al. (2025) under CC BY license. Both groups demonstrated their sensors in...in a variety of neuronal imaging applications, including confocal and widefield imaging, two-photon microscopy...

...Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage...receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor (AAVR)2   AAV2 Heparan...Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...(PDGFR) α and β3 AAV6 α2,3-/α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan (HSPG)3 AAV...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by...

...amino acids (ncAA) in a protein of interest. Applications By making small changes in selected amino acids...pNEU-hMbPylRS-4xU6M15 PylRS M. barkeri Pyl-like click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644...Bacterial TAG Lital Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta...Davis L. & Chin J. W. (2012). Designer proteins: applications of genetic code expansion in cell biology . ...

...between mGold2s and mGold2t depending on the application. Figure 1: Super-resolution imaging...from Lee & Lai et al. (2025) under CC BY-NC-ND license. If you’re currently using mVenus or mCitrine...proof-of-concept, they believe that the strategy will be applicable with other TnpBs and in other plant species, ...available for tracking cell lineage, different applications require careful selection or design to ensure...Reproduced from Singh et al. (2025) under CC BY license. Libraries are available with T-Sapphire, ...for gene therapy and ex vivo cell engineering applications and is especially well-suited for lung cells...tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV serotypes...

...suppliers, and public health and medical organizations are working to help educate the public about proper...containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving...essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such...mammalian viral vectors in that the vectors are replication-deficient. Thus, a very large number of phagemids...CRISPR antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...

...for both transient and stable gene expression applications in plants. This smaller, easily customizable...Agrobacterium are termed binary vectors because they must replicate in two species: Agrobacterium and E. coli. Like.... coli. However, they also need to be able to replicate in Agrobacterium to ensure that they are maintained... Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium, along...bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they...constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized in studies...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...

...to disparate physiological effects and complicate any metabolic study. The Ralser lab has created a unique.... Seeliger MA, Young M, Henderso MN, Pellicena P, King DS, Falick AM, and Kuriyan J. Protein Sci. 2005...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...and ubiquitous reporter assay. In their recent publication, Kroeze et al. describe their enhanced TANGO .... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...

...emission wavelengths, which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These... substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby...typically bind to. Stability of fluorescence: For applications that involve ranges of pH, and significant lengths...light, is another factor to consider. If your application requires longer periods of exposure, you’ll want...have shown that different fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to ...descriptions, or technical manuals. Specific applications and matching dye/ligand choices For effective...beyond the diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific...

...for your application. Image reused form Doman and Pandey et al., 2023 under CC-BY 4.0 License. Find... CreVLPs, and eVLPs for in vivo and in vitro applications.   Figure 2: Modular features of DIRECTED...Strebinger et al., 2023, reused under CC-BY 4.0 License. Importantly, even commercial antibodies can...approach for experimental therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D...interactions or map a local proteome, but some applications have been challenging or inaccessible. The Ting...alkyne-phenol, to label proteins with an alkyne group for click chemistry reactions instead of biotin — a valuable...from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0 license. This system provides a unique new way to record...

...list of individual screens curated for the publication. Clicking on the screen name takes users to a display...favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section...manually curated and annotated from more than 45 publications, beginning with the seminal research articles... makes it possible for researchers to query all public CRISPR results for any gene of interest. In addition...screen curated in ORCS is linked to the original publication via its PubMed identifier, which can also be ...screens of interest.   Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary... supplemental data tables available with the publication are also provided for the user. This approach...

...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and... In a column-based approach, the substrate is a silica matrix fixed in a column and you flow lysate and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference...

...Depending on the research question and downstream application, one or several of those techniques can be used...your flow analysis. However, certain downstream applications, like an in vivo adoptive transfer of those ...available fix/perm kits allow for labeling of cytosolic proteins, as well as proteins of the secretory...intranuclear proteins of interest, you can label cytosolic proteins alongside the intranuclear ones. You ...You do not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...or free amines of proteins. Depending on the application, they can be membrane-permeant or membrane-impermeant...are positive or negative for the dye. The main applications of dye stains in flow cytometry are cell proliferation...

...what we do not understand, and the complicated and unique metabolic processes of plants are being investigated...involved in plant synthetic biology. Challenge #1: Public perception of synthetic biology One of the biggest...difficulties that plant biotechnologists face is public perception of genetic modification (GM) and synthetic...benefits and future potential of the technology to the public in an open, responsible manner. At Revolution Bio...obstacles to plant synthetic biology In addition to public resistance, an overall limited understanding of...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools... intellectual property concerns – patents and licensing agreements. Developing a product without the use...

...constitutive splicing studies and has been optimized for high efficiency splicing. If intron splicing does not...potential applications of CRISPR/Cas9 in genome-engineering. One particularly attractive application involves...in order to avoid complicating effects from global toxicity due to loss of splicing. To this end, destabilizing... system widely accessible for many potential applications. Polstein LR & Gersbach CA, Nat Chem Biol ...amenable to both high and low throughput screening applications. The system can be readily modified to use different...cloning platform for imaging and flow cytometry applications Designing customized fluorescent reporters can...luciferase reporter system for detection of pre-mRNA splicing   The Gideon Dreyfuss lab has developed rapid-response...