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We narrowed to 237 results for: Mammalian expression plasmids

Showing: 161 - 180 of 237 results

  1. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Addgene blog:  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...process known as plasmid multimerization. Plasmid Multimerization Early studies on plasmid topology recorded...ligate the plasmid, and re-transform the ligated plasmid to recover the monomeric form of the plasmid. Have... C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells. J Gene... monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The plasmid can exist on... have been utilizing full plasmid sequencing services to verify the plasmids, including long-read sequencing... the sequencing results for their plasmids, they found the plasmids were much longer than expected, and...

  2. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...Designing the donor plasmid Figure 2: Donor plasmid design strategy. A donor plasmid contains 1 kb homology...found within the homology areas of the plasmid to prevent the plasmid from being cut and destroyed by CRISPR... the tag. Created with BioRender.com. Plasmids, plasmids everywhere To find out more about gene edited...expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag a wide...wide variety of genes with GFP. These plasmids are available through Addgene, and the stem cell lines we...found in the Cell Catalog on our data portal. The plasmids should be functional in many different human cell...constructs below. Read on to find out how these plasmids work and to discover more resources for your genomic...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...neuronal connectivity in the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors...transgene expression. Fluorescent proteins are widely used for tracing and their expression is often made...Cre-dependent vectors are used in transgenic animals that express Cre in only specific cell types, for example only...labelling the subset of infected cells that also express Cre. Another advantage of AAV vectors is that there...et al. 2014 traced neurons with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection...Injection into a conditional mutant mouse brain that expressed Cre recombinase only in pyramidal cells of the...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California...

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Differential cytosine editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were ...seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly edited U bases...Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature...Additional resources on Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...Developments in mammalian and yeast cells using modified Cas9 proteins to regulate gene expression (CRISPRi/...step as simple as heat-shocking to induce the expression of a Cre recombinase contained within the knock-in.... elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript... C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives It is interesting to...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...pCEP4CyPet-MAMM - Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1...Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian Expression AcGFP1 475 505 28 Prone ...sfGFP-N1 - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP ...pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression...Topaz-N1 - Mammalian Expression Topaz-C1 - Mammalian Expression Topaz-pBAD - Bacterial Expression mTopaz ...

  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...for mammalian edits is to ensure the editors make it into the nucleus and that they are expressed well...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...editor with improved nuclear localization and expression. This base editor was named ABEmax. In 2020, ... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...ArcBe4max, ABEmax   Improved nuclear localization and expression Gaudelli et al., 2017 Adenine base editors ...Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

  8. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...requires expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...maintained by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection...pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none...BPK2301 65778 Mammalian BsmBI none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none...

  9. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene ...pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with...pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with ...fluorescent protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine with...Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses...N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus...with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression... Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2...Species-Specific Expression If you want to drive expression of your favorite gene, you will need a plasmid with...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...translation for mammalian expression pDest-527 - N-terminal His6 tag for bacterial expression (Gateway) pYD1...

  11. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...for expression of a “+” heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells...for expression of a “-” heterodimeric FokI domain. Miller et al., Nat Biotech 2007 ) in mammalian cells..., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of a ZFN targeted to a ...

  12. Genetic Code Expansion

    Type
    Collection
    ... you will need two plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate...guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian cells. Plasmid...often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place where...different amino acid. They are typically expressed from a single plasmid, with multiple copies of the tRNA. ...Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian cells....Research Center . Browse Synthetase Plasmids The table below highlights plasmids that contain aminoacyl tRNA ...synthetase for use in E.coli and Mammalian Cells. Many of the plasmids also contain one or more copies ...

  13. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...separate gRNA expression plasmid to target the dCas9-repressor to your specific locus. ID Plasmid Gene/Insert...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function...your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN, both...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...CRISPR prime editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type...Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila ID Plasmid Gene/Insert...Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  16. Luciferase Plasmid Collection

    Type
    Collection
    ...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ... preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... into a luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence...

  17. Antibody Plasmid Collection

    Type
    Collection
    ...antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or... into a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant ... Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant...partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large proteins made...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...tags, such as GFP, may interfere with protein expression or function in live cells. Fluorophore properties...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...techniques to study mitochondrial dynamics in mammalian cells. Biophysics Reports, 3(4–6), 64–72. https...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...

  19. Arf GTPase Family

    Type
    Collection
    ... Richard Kahn Lab Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor...examples of plasmid types available for each gene. Click the gene name for a full list of plasmids available... have plasmids available for request. Family Gene Entrez Gene ID Length (aa) Example Expression Systems...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Promoters Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection...endogenous mammalian transcription factors to further minimize background expression. Improved expression of ...Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection. See Plasmid #21916 for ... other viral tTA plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the ...dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9...B19G ( Plasmid #100799 ). Ian Wickersham 172878 pSF3-ultraID Expression of UltraID in mammalian cells ...gene expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...
Showing: 161 - 180 of 237 results