We narrowed to 563 results for: PAC
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TypeBlog Post...variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel...does not, be sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction...Aggregation and storage Aggregation of antibodies may impact their quality and efficacy (Wang et. al., 2018)...10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to ...
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Getting to Know Expansion Microscopy
TypeBlog Post...individual presynaptic terminals, which are jam packed with everything needed for synaptic transmission...? ExM provides a straightforward answer to the space problem. Instead of imaging smaller, what if we ...the anchoring, digestion, and expansion steps can impact the integrity of different targets and labels. ...continuity can appear reduced in ExM, which could impact how you interpret a target’s structure. For example... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog Post... plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey... The plasmid's journey begins First, each package is opened and the contents are carefully inspected...deposits arrive from labs around the globe. The packages have spent days to weeks in transit and some have...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many... -
Technical Design of a Western Blot
TypeBlog Post...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...conjugate will have to be tested to ensure it doesn’t impact the antibody’s binding to your protein of interest... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well for proteins... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described ...should be screened for activity in vitro and then packaged into AAV for in vivo delivery. Multiplex gene ... also possible and is only constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG. If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead. Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... -
Bioinformatics at Addgene
TypeBlog Post...complete package, all of the pieces of software that depend on each other for the entire package to run....in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...differing customs processes, and the distances the packages needed to travel meant the accessibility varied...such as using couriers that refill dry ice if the package stays in transit for a certain number of days and...distributor who, among other things, could ensure our packages navigated customs and arrived at their destination...for tools such as empty backbones, lentiviral packaging systems, and luciferase expression. On individual... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...lentiviral infection begins with your cells, both your packaging cells and your target cells. Poor tissue culture...harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy...benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest...density, volume, incubation time, and MOI can all impact the success of your lentiviral infection. To ensure... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...take up essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods...the chosen acceptor vector. The vectors are then packaged into AAV virions via transient transfection of...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a... cell types not of interest. Systemic capsids packaging Cre-dependent transgenes can also be paired with...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use...to be administered concurrently to minimize the impact of neutralizing antibodies developed in response...promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 ...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,... -
Finding Your Science Policy Path
TypeBlog Post...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...webinar via the NC Science Policy student group SciPolPack for a more detailed discussion on how to to define...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...her work focuses on incentivizing the real-world impacts of health services research. She is particularly... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia. Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...–296. https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu, D. R. (2015). Methods for the directed... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact in terms ... product? How can we consider the environmental impact of those processes? Who even regulates them? You... -
What's New in CRISPR - December 2019
TypeBlog Post...lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression...aptamer which binds an aptamer binding protein for packaging into a lentivirus-like particle. Read the paper...paper in Nucleic Acids Research Find the gRNA and packaging plasmids Cytosine deaminase for RNA editing... -
ReFigure: Save Scientific Figures into Dashboards and Share Your Insights
TypeBlog Post...this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge and ...definitely give you credit for it and increase your impact! Download ReFigure extension and visit ReFigure.org... with Joe Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete... -
Advice for Choosing a Research Project
TypeBlog Post...the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests...when selecting a research project. What will the impact be in a best case scenario? If you get your dream...what will your paper look like? What will be the impact on your field? You want to choose a research question... -
Immunology Research Plasmids and Resources
TypeCollection...polypeptide 1 (pituitary) receptor type I PAC1, PACAPR, PACAPRI ADIPOQ adiponectin, C1Q and collagen domain...receptor 1 FLJ41949, HVR1, II, PACAP-R-2, RDC1, VAPC1, VIPR, VIRG, VPAC1, VPCAP1R VIPR2 vasoactive intestinal...cell types including T cells and B cells. It also impacts the development and function of innate immune cells...intestinal peptide receptor 2 FLJ16511, VPAC2, VPCAP2R XCL1 chemokine (C motif) ligand 1 ATAC, LPTN, LTN... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...around the world were closed and not receiving packages. So the entire team mobilized to figure out how...subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene Team because...workforce for when we need to be working at full capacity to serve the scientific community. First, we applied...