Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 497 results for: RAP

Showing: 161 - 180 of 497 results

  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...serotype also has promising therapeutic applications; it can enhance therapeutic delivery across broader ... have identified 180 brain regions. Both this geography and the way the brain is connected to itself across...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...previously a large barrier for gene therapy. In fact, AAV gene therapies have already been approved in the...Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure out How to Get the Most from Your...

  4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome replication more...types of fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before...T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization of gene dosage in E. coli using DIAL...

  6. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...art with colorful microbes? Try your hand at photography and participate in a science photo competition...Radio, WIRED, Scientific American or National Geographic. Similarly, media fellowships from the British...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...pot.’   Figure 1: Single-Pot Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA ... single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR ...

  8. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...type of article. Now, search results display a bar graph of the number of papers with your search term published...several ways to focus your search results. A bar graph of the number of papers with your key term published...blog posts on free tools for making scientific graphics post and social media for scientists ...

  9. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...Vivo Persistence after Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe.2005.06.003...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org/10.1038...

  10. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...5’-phosphorylated ssDNA guides, which might be rapidly degraded by the cellular machinery, leaving a small...system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise if someone came up...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh...

  11. DNA Purification Without a Kit

    Type
    Blog Post
    ... cellulose also efficiently binds, or at least entraps nucleic acids. The Botella lab took advantage of... References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA Purification Method Using...Peng, F., Huang, L., Zhao, S., & Deng, W. (2018). Rapid Regeneration and Reuse of Silica Columns from PCR...

  12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... the foundation of modern drug discovery and therapeutics. We’ll have more to say about clinical applications...apply this knowledge to create targeted disease therapies with minimal side-effects on healthy cells and... a 3D Fractal Figure 2: DNA compaction. Graphics courtesy of beyondthedish (left) and biobabel ...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...number of phagemids would be needed for an in vivo therapy. Another strategy for CRISPR antimicrobials combines...replication-competent phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics... phage therapy...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...lateral flow strips are easy to transport and work rapidly, providing reliable results in as little as an ...diagnostics (e.g. pathogen or virus detection), therapeutics and sensitive genotyping. The beauty of SHERLOCK...within one hour. DETECTR thus has the ability to rapidly detect nucleic acids with high selectivity and ...

  15. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...other large research institutions. The following graphic depicts the ways in which the two guiding principles...it difficult or impossible to reproduce. As the graphic depicts, RSpace supports linking to all of the ... other tools and resources. As depicted in the graphic, RSpace is integrated with most of the tools noted...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...(2024). Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders...interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal background...2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium...

  17. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...of the sample to that of a standard curve to extrapolate the concentration of antigen in the sample. A... present in the sample and is calculated by extrapolating from the standard curve. Because the primary... present in the sample and is calculated by extrapolating from the standard curve. Scientists often opt...

  18. Hot Plasmids - November 2023

    Type
    Blog Post
    ...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called...specific cell types. It can also make CAR-T cell therapy more accessible by simplifying the production process... offers a promising approach for experimental therapies and medical applications.  Find DIRECTED plasmids...

  19. Viral Production at Addgene

    Type
    Blog Post
    ...vector particles.     Figure 3: Electron micrograph of AAV vector preparation shows that the vast...genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089...a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...closing. Parts of a cover letter The opening paragraph is the place to introduce yourself and your background...body of the cover letter should be one to three paragraphs. This is where you make the case for why you ...questions the hiring manager may have. The closing paragraph should again show your enthusiasm for getting ...
Showing: 161 - 180 of 497 results