We narrowed to 202 results for: cycs

...to subject your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5...

...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...

...is present, the probes will ligate. Subsequent cycling through rounds of melting and annealing allows ...

... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...

...presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

...modifications, determining cell health, analyzing cell cycle stages, and detecting specific populations of cells...

... depth, the Jennifer Doudna lab maintains an encyclopedia of Class 2 CRISPR systems called CasPEDIA, with...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

...harmful mutations More, due to selection from more cycles of proliferation Less Cell types All types Less...

...many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et...

...reversible and repeatable when blue light (450nm) was cycled on-off-on. Using a slit photomask, the researchers...

...replicates in the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s ...

...processes such as intracellular trafficking or cell cycle dynamics. Protein Excitation (nm) Emission (nm) ...

...cells and to the uncoating step in the viral life cycle. Using AAV for neuronal tracing AAV vectors are ...

...least 10 µL. Antibodies cannot tolerate freeze-thaw cycles. Here are a few tips for storing your antibody:...

...122650 Mm-PylRS-AF/Pyl-tRNACUA PylRS M. mazei trans-cyclooct- 2-ene-lysine (TCOK) Mammalian TAG Howard Hang...

...stripping, since you’ll lose protein with every strip cycle. Troubleshooting the troubleshooting There’s many...

...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters. Cycling Step Temperature...Droplet Digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing...

...sealed, proceed to thermocycling. Thermal Cycling Run the following PCR parameters: Cycling Step Temperature...Droplet digital PCR System, Bio-Rad, DX200 Thermal Cycler, Bio-Rad, T100 PCR Plate Sealer, Bio-Rad, PX1 1...Temperature (°C) Time (min) Ramp Rate (°C/sec) # Cycles Enzyme Activation 95 10 2 1 Denaturation 94 0.5 2 40 ...

... heating and cooling (denaturing and annealing) cycle. The process became automated with the discovery...polymerase can withstand many heating and cooling cycles, which would denature DNA polymerases from other...a few nanograms of template DNA through several cycles of denaturation, annealing, and synthesis. After... 2 minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. Run 2 μL...