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...or whole animals presents unique challenges. Genetically-encoded fluorescent proteins most suitable for...opens in a new window) or plasmids' original publications. Far-Red Protein Excitation (nm) Emission (nm...

...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced...sites and then generates individual user-friendly graphical roadmaps for each target that guide the user through...

...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast...

...of action, regulation and post-translational modifications, and metabolic pathways and protein interactions...His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map, a TEV cleavage site is present...

... below. Color is used for clarity and does not indicate a specific relationship. The content for this ...Pathway Color is used for clarity and does not indicate a specific relationship. The content for this ...

...Chemogenetics AAV Use our chemogenetics AAV to chemically induce neuronal activity in specific cell types...Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9, PHPeB Jordane Dimidschstein...

...Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. ...consortium of biologists and computer scientists dedicated to providing the research community with accurate...

...Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the ...: 28814507 (Link opens in a new window) More publications from the Allen Institute for Cell Science (Link...

...well as applied research in small biotech and pharmaceutical companies. The RESOLUTE project has received...interactions represent the framework that provides biological context. Assays monitoring impedance, fluorescence...

...activator-like (TAL) effectors, scientists in several biological disciplines are coming closer to this DNA targeting...DNA regions into a single construct would be technically challenging. Thanks to efforts by the Bogdanove...

... enzyme that cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains...Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor . Cell, 181 (2), 271...

...with light. Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See...

...opens in a new window) or plasmids' original publications. Note for all tables: Brightness is the product...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

...deposited plasmids include: Joan Brugge , Harvard Medical School Klaus Hahn , UNC Richard Hynes , MIT Rick...

...Xenopus". Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

... Plasmid Gene/Insert Vector Type Promoter PI Publication Return to top CRISPR Resources Addgene has a ...

... acting as an integration point for multiple biochemical signals. In collaboration with numerous research...

...the three dimensional structures of proteins of medical relevance, and place them in the public domain ...

...Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR Monosynaptic Neuronal...

...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...