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We narrowed to 562 results for: pac

Showing: 161 - 180 of 562 results

  1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...lost during fixation with these chemicals, which impacts sample structure and could also cause your target...destroy it for others. That said, organic solvents’ impact on lipids means that they are able to permeabilize....   Figure 1: Choice of fixation method impacts antibody binding. Formaldehyde cross-links the ...detergent has different chemical properties that will impact the level of permeabilization and membrane integrity...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...individual presynaptic terminals, which are jam packed with everything needed for synaptic transmission...?  ExM provides a straightforward answer to the space problem. Instead of imaging smaller, what if we ...the anchoring, digestion, and expansion steps can impact the integrity of different targets and labels. ...continuity can appear reduced in ExM, which could impact how you interpret a target’s structure. For example...

  3. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ... plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the journey...    The plasmid's journey begins First, each package is opened and the contents are carefully inspected...deposits arrive from labs around the globe. The packages have spent days to weeks in transit and some have...barcode can store a lot of information in a small space and is ideal for tracking large collections. Many...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...binding capacity and high protein retention. It comes in a variety of pore sizes and protein capacity, allowing...conjugate will have to be tested to ensure it doesn’t impact the antibody’s binding to your protein of interest... is a little quicker to use, with high binding capacity, though it has lower retention after binding and...10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well for proteins...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described ...should be screened for activity in vitro and then packaged into AAV for in vivo delivery. Multiplex gene ... also possible and is only constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes...

  6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ...complete package, all of the pieces of software that depend on each other for the entire package to run....in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...differing customs processes, and the distances the packages needed to travel meant the accessibility varied...such as using couriers that refill dry ice if the package stays in transit for a certain number of days and...distributor who, among other things, could ensure our packages navigated customs and arrived at their destination...for tools such as empty backbones, lentiviral packaging systems, and luciferase expression. On individual...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lentiviral infection begins with your cells, both your packaging cells and your target cells. Poor tissue culture...harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy...benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest...density, volume, incubation time, and MOI can all impact the success of your lentiviral infection. To ensure...

  10. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...editing capabilities but are small enough to be packaged in AAV particles. Want more information on the...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...take up essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods...the chosen acceptor vector. The vectors are then packaged into AAV virions via transient transfection of...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with...

  12. Plasmid Collections

    Type
    Collection
    ...Kits Kits are groups of plasmids that have been packaged together because they are intended for use as ...Migration Consortium Center for Genome Engineering Impact of Genomic Variation on Function (IGVF) Consortium...

  13. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares), and also...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a... cell types not of interest. Systemic capsids packaging Cre-dependent transgenes can also be paired with...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use...to be administered concurrently to minimize the impact of neutralizing antibodies developed in response...promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 ...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

  15. Finding Your Science Policy Path

    Type
    Blog Post
    ...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...webinar via the NC Science Policy student group SciPolPack for a more detailed discussion on how to to define...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...her work focuses on incentivizing the real-world impacts of health services research. She is particularly...

  16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia.   Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination...

  17. p53 Pathway

    Type
    Collection
    ...weight (53 kDa) when run on an SDS-PAGE gel. Cancer Impact p53 is the most frequently mutated gene (>50%) ...disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function...

  18. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact in terms ... product? How can we consider the environmental impact of those processes? Who even regulates them? You...

  19. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression...aptamer which binds an aptamer binding protein for packaging into a lentivirus-like particle. Read the paper...paper in Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing...

  20. Viral Production

    Type
    Collection
    ... Viral Vector Packaging Service Viral Production Viral Production Jump...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture...
Showing: 161 - 180 of 562 results