We narrowed to 14 results for: AAVS1

...Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-...mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of ...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405 ...

...design and delivery. They generated hPSC lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB...

...targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe ...cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 ...Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector...vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging...

...situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest gRNAs had cleavage sites ...

...also aids in infrequent genome integration at the AAVS1 locus in humans. Cap is a structural capsid protein...

...action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging...

...editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment they ...

... Ward 105840 pUCM-AAVS1-TO-hNGN2 Introduction of dox-inducible human NGN2 into AAVS1 safe harbor site,...of GFP T2A Cre recombinase. Floris Foijer 165457 AAVS1-Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of...degron system Masato Kanemaki 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Tet-inducible expression gene of ...

...though it can integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified ...

...advantageous to adapt PITCh to insert genes into AAVS1, the “safe harbor locus” of the human genome, as...

...integrating into the host genome at a specific site, the AAVS1 site on human chromosome 19. This integration is...Rep-binding elements present in the AAV ITRs and the AAVS1 site. Since the Rep region is removed from the rAAV...

...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available...

...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

...SLC1A3 Episodic ataxia Giulio Superti-Furga 132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT GFP TREtight Parkinson's...Parkinson's, FTD Gerold Schmitt-Ulms 132393 AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP MAPT GFP TREtight Parkinson's...