Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
Type
Blog Post
... tested by Quadros et al.
When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend...pups born, with typical efficiencies of 30-60%. Quadros et al. attribute much of this variation to differences... locus may also influence efficiency. However, Quadros et al. saw success with Easi-CRISPR across three...Channabasavaiah B. Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with editing this blog post.
References...PMID: 26242611 PubMed Central PMCID: PMC4525291
Quadros, Rolen M., et al. “Easi-CRISPR: a robust method...Central PMCID: PMC5434640
Miura, Hiromi, Rolen M. Quadros, Channabasavaiah B Gurumurthy, and Masato Ohtsuka...