We narrowed to 53 results for: BID
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TypeBlog Post... two global stock centers for Arabidopsis thaliana (Arabidopsis). Our mission is to collect, preserve,...in 60 countries each year. Introducing Arabidopsis Arabidopsis is an important model system for research...Translating Research on Arabidopsis Into a Network of Educational Resources). Arabidopsis is used in research... the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for...Ohio State University. Established in 1991, the Arabidopsis Biological Resource Center (ABRC) is one of two...distribute seeds, DNA and other resources for Arabidopsis and related species. Located at The Ohio State...globe. As a member of the Brassicaceae family, Arabidopsis is related to a variety of food crops such as...
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Tips for arabidopsis transformation
TypeBlog Post..., a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons... are many motivations to generate transgenic Arabidopsis, from studying transcriptional and translational... plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation...transformation protocol, as well as protocols for growing Arabidopsis are also available (Weigel and Glazebrook, 2002...vector is the first step in generating transgenic Arabidopsis. There are a bunch of great options, but here... pool you will be selecting from later. The Arabidopsis transformation process The process itself is ... the chances of tDNA integration. Selecting Arabidopsis transformants Once your seeds from your T0 plants... -
p53 Pathway
TypeCollection...angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid BH3 interacting domain death agonist CASP3 Caspase... -
Five Popular Model Organisms, Part 2
TypeBlog Post...is as a biomedical model organism. Arabidopsis thaliana Arabidopsis thaliana is one of, if not the most...community. The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research...characterized genome, and widespread utilization, Arabidopsis has made significant contributions to understanding...understanding plant and crop science alike. Arabidopsis has many of the traits that make an ideal model organism...maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms is its...research development. Arabidopsis thaliana was fully sequenced by the year 2000, becoming the first plant...’s function. Some key genetics research with Arabidopsis include understanding of allelic variation between... -
RUBY-Red Siliques
TypeBlog Post...occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR...planning to use it to simplify my work with an Arabidopsis mutant that can’t set seeds. I study the development... Fig. 3: (Top right) A RUBY-expressing Arabidopsis seed following floral dip transformation next...without antibiotics. (Top left) Mature silique (Arabidopsis fruit) with fertilized developing seeds from ... the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: CRISPR-Mediated Plant... -
Antibody Validation for Flow Cytometry
TypeBlog Post... ** = recombinant antibody. Figure adapted from Biddle et al., 2024. Once an antibody is found ...Antibodies, with significant input from Michael Biddle, PhD, also from Only Good Antibodies. The Only ... and resources References Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. ...12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., Fotouhi, M., Jones, C., Ayoubi...https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., Smallwood, D. T., Weston, C. A., Castells...doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston, C.... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post... C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene function in C. roseus...method for overexpressing genes In plants like Arabidopsis and tobacco, Agrobacterium infiltration into ...expression assay by agroinfiltration of leaves." Arabidopsis Protocols. Humana Press, 2006. 225-229. PubMed...transient gene expression analysis in seedlings of Arabidopsis and other plant species." Plant methods 5.1 (...of our plant biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...functions in multiple species, including tobacco and Arabidopsis. Improving Agrobacterium binary vectors Vectors...domains and characterized in studies of tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and...efficiently inherited in both transgenic tobacco and Arabidopsis. Finally, Sahoo et al. demonstrate that this ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post... specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that the ...induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she...salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression patterns in plants with enhanced... -
All in a Twist: dsRNA
TypeBlog Post...different mechanism thought to be associated with bidirectional transcription. In an uninfected cell, dsRNA ...interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted repeats. These ...genomes which can produce dsRNA as a result of bidirectional transcription of their circularized DNA. These... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...expression patterns with reprogramming outcomes (Biddy et al., 2018). Tracking lineage and cell identity...tool. Check out the CellTag webpage! References Biddy, Brent A., et al. "Single-cell mapping of lineage... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...about Synthetic Biology Find Arabidopsis educational resources at the Arabidopsis Biological Resource Center... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they call the LADL Anchor. Using... iterations is the addition of CRY2, another Arabidoposis protein that heterodimerizes with CIBN upon ... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate particular... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...multi-cloning site flanked by T7 promoters to allow for bidirectional transcription to create dsRNA. Students can ...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ... -
Plant Plasmids and Resources
TypeCollection...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (...tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of...in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more...Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with...designed for gene silencing in plants: microRNAs in Arabidopsis - Detlef Weigel. microRNAs in rice - Detlef Weigel... Weigel. microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system...Tools for an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens... -
Hot Plasmids: Winter 2025
TypeBlog Post... to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable...enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...amiRNAs based upon miRNA precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA...plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018... -
Retrograde AAV viral preps
TypeCollection...CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional, Cre-dependent...Dopamine Optogenetics Activators Inhibitors Bidirectional DREADDs Activators Inhibitors Other Molecular... -
27 Hot Plasmids from 2016
TypeBlog Post...allow for cloning of up to two genes with a bidirectional promoter, integrate at 11 specific chromosomal...up to 120 kb) deletions in N. benthamiana and Arabidopsis. The authors found that deletion efficiency was...