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We narrowed to 29 results for: ChR2

Showing: 1 - 20 of 29 results

  1. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...intensity than existing channelrhodopsins CoChR and/or ChR2(H134R). Designer channelrhodopsins paired with ...injections. When packaged in PHP.eB, ChRgers and ChR2(H134R) both reach the brain, but individual neurons...multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because it’s lower conducence... a burst of light that could activate ChRger2 or ChR2(H134R) when they poked their nose into a hole in...what was seen with ChRger2 expressing mice, while ChR2(H134R) expressing mice did not have this self-stimulation...systemic delivery with PHP.eB compared to the existing ChR2(H134R).  In the second experiment, ChRger2 was delivered...the right M2 are activated, but mice that received ChR2(H134R) did not turn. Left-turning behavior in ChRger2...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon...Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre...have expanded our AAV collection to include two new ChR2 constructs that allow for targeted expression. Plasmid...available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry reporter. We now also...AAVrg for plasmid 83898, which targets expression of ChR2 to forebrain GAB-ergic interneurons through the ...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...      In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse... light-gated cation channel, Channelrhodopsin 2 (ChR2), exists in several states: closed/light receptive.... If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity to...

  5. Optogenetics AAV Preps

    Type
    Collection
    ...collection. Opsin Excitatory Wild-type ChR2 ChR2/H134R soCoChR ChR2/other mutants Chronos, Chrimson, ChRmine...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell...mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Fishell 135634 pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive...Foff 2.0-ChR2-mCherry nEF ChR2 mCherry Cre dependent 8 Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry...mCherry nEF ChR2 mCherry Flp dependent 8 Deisseroth 137163 pAAV-nEF-Con/Foff 2.0-ChR2-EYFP nEF ChR2 EYFP Cre...pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry CAG ChR2/H134R mCherry Flp dependent 1 Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R...Syn ChR2/H134R GFP Constitutive 8, rg* Boyden 100054 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 CAG ChR2/H134R...

  6. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent ChR2 Gradinaru...BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4_ChR2_mCherry BiVIPe4 Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3... Activator ChR2 Fishell 213941 pAAV_BiPVe3_ChR2_mCherry BiPVe3 Activator ChR2 Fishell 213945 pAAV_BiSSTe4...Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein 214846 AiP13755...pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) minBG Activator ChR2(H134R) AIBS , Ting...pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA...213815 pAAV_BiSSTe10_ChR2_mCherry BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC projections...Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates neurons...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP Cre AND Flp FRT/F5 Yes 137141 pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP Flp ...137142 pAAV-nEF-Con/Fon-ChR2-mCherry Cre AND Flp Yes 137143 pAAV-nEF-Con/Foff 2.0-ChR2-mCherry Cre AND NOT...pAAV-nEF Con/Foff hChR2(H134R)-EYFP Cre AND NOT Flp F3/F5 No 137163 pAAV-nEF-Con/Foff 2.0-ChR2-EYFP Cre AND...pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP Flp AND NOT Cre No 137139 pAAV-nEF-Con/Fon-ChR2(ET/TC)-EYFP Cre AND...proof-of-concept targeting approach in 2014 1 (using EYFP and ChR2-EYFP as payloads). This approach has been broadly...experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number of additional, validated molecular...NOT Flp FRT/F5 Yes 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry Flp AND NOT Cre Yes 137145 pAAV-nEF-Con/Fon-bREACHes-EYFP...

  10. Brain Initiative Collection

    Type
    Collection
    ... Gordon Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain... Gordon Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain...Gordon Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain...PHPeB pAAV-CAG-DIO-ChR2(H134R)-eYFP Cre-dependent AAV expression of humanized ChR2 (with H134R mutation... 135634-AAV1 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical ... 135634-AAV9 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical ...135634-PHPeB pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical ...

  11. Optogenetics Guide

    Type
    Guide
    ...reinhardtii ChR2 Widely used light-gated cation channel from Chlamydomonas reinhardtii (CrChR2) 470 ChR2/H134...light activated cation channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance at physiological... C1C2 ChR1-ChR2 chimera 470 ChIEF, ChEF, ChD Engineered by chimeragenesis of ChR1 and ChR2 450-470 C1V1...mouse line, such as for example the VGAT-ChR2 mouse, where ChR2 is expressed under the control of the vesicular...into the cell when stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is ...include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ChIEF Increased channel (on/off... aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus expressed in all GABAergic neurons. In this...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...controlling neuronal activity, channelrhodopsin-2 (ChR2), a blue light-activated cation channel, from the...opsin.      Figure 2: Diagram of excitatory (ChR2) activation by light showing ions flowing with the...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... viral prep AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) AAV-PHPeB...Control Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell ...Control Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell ...Control Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell ...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...AAV-FLEX-rev-ChR2-tdtomato Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics...-AAV5 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda...AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-9-18917P 18917-AAV9 AAV-FLEX-rev-ChR2-tdtomato Scott...pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R...Addgene ID Name PI AV-1-18916P 18916-AAV1 AAV-FLEX-rev-ChR2(H134R)-mCherry Scott Sternson AV-1-20949P 20949-...Douglas Kim AV-10-18917P 18917-AAV1 AAV-FLEX-rev-ChR2-tdtomato Scott Sternson AV-10-PV1963 105542-AAV1...pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-...

  16. Optogenetics Plasmid Collection

    Type
    Collection
    ...widely used Channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens...

  19. Brain Armamentarium

    Type
    Collection
    ...pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) Expression of ChR2(H134R)-EYFP in striatal...Jonathan Ting Viviana Gradinaru 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged...Fishell Viviana Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged...Fishell Viviana Gradinaru 213937-PHPeB pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged...Fishell Viviana Gradinaru 213915-PHPeB pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged...Fishell Viviana Gradinaru 213856-PHPeB pAAV_BiVIPe4_ChR2_mCherry AAV construct expressing mCherry-tagged...Fishell Viviana Gradinaru 213830-PHPeB pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    ...hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find these AAVs at Addgene ...
Showing: 1 - 20 of 29 results