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  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...hybrid mammalian promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin... trying to pick your perfect promoter! Eukaryotic Promoters   Promoter   Primarily used for RNA     ...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...activate promoter. Ac5 General expression mRNA Strong insect promoter from Drosophila Actin 5c gene .... In practice, the term "promoter" describes the combination of the promoter (RNA polymerase binding site...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters. This ...

  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ...from the CMV promoter pAdx-CMV-YFP 73348 Express YFP with an NLS from the CMV promoter pAdx-CMV-dsRed 73349...gene from the CMV promoter pCDH-CMV 72265 Express gene of interest from the CMV promoter pCDH-EF1 72266...from the CMV promoter pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...Xgal/IPTG plate pAdx-CMV-copGFP 73346 Expresses copGFP from the CMV promoter pAdx-CMV-tdTomato 73347 Expresses...73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA...phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest from the CMV promoter. WPRE has been ...

  5. Cre-lox system

    Type
    Collection
    ...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII...27493 pBT140 Cre CMV Mammalian Luo 27546 pLM-CMV-R-Cre Cre and mCherry coexpression CMV Lentiviral Sadelain...Lentiviral Oka 73350 pAdx-CMV-iCre-P2A-copGFP iCre and GFP CMV Adenoviral Oka 73351 pAdx-CMV-iCre-P2A-tdTomato...Description Promoter Expression System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM ...pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP/CREM floxed RFP within CREM CMV Mammalian Green 8403 p153...CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian...split-Cre CMV Mammalian Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian ...

  6. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ... that physically interact with the Pax5 promoter in a chicken B cell line. Fujita T, Kitaura F, Yuno M...Expresses a guide RNA (gRNA) to target human IRF-1 promoter 61080 gRNA-hIRF-1 #12/pSIR Expresses a guide RNA... RNA (gRNA) to target human IRF-1 promoter 62190 3xFLAG-dCas9/pTEF1p-CYC1t Expresses 3xFLAG-dCas9 in budding...expressing dCas9-2xAM and gRNA for human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses ...Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system. 105283 hIRF-1 gRNA #351...Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system. References An enChIP system...:387. doi: 10.1186/s13104-018-3486-3. PubMed . Promoter-associated proteins of EPAS1 identified by enChIP-MS...
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