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We narrowed to 17 results for: Cre-dependent ChR2 expression

Showing: 1 - 17 of 17 results

  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo...serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain GABA-ergic interneurons...available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry reporter. We now also...GFP dependent Cre. The two segments come together in the presence of GFP to form a functional Cre enzyme...preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing SARS-CoV-2 proteins ...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...collection to include two new ChR2 constructs that allow for targeted expression. Plasmid 75470 is now available...

  2. Caltech Systemic Capsids

    Type
    Collection
    ...105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression...Activator, Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD...Activator ChR2 Fishell Recombinases 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...BiCHATe27 Activator ChR2 Fishell 213856 pAAV_BiVIPe4_ChR2_mCherry BiVIPe4 Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3...

  3. Brain Initiative Collection

    Type
    Collection
    ...PHPeB pAAV-CAG-DIO-ChR2(H134R)-eYFP Cre-dependent AAV expression of humanized ChR2 (with H134R mutation...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization...-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...cells expressing Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp.../Fon-ChR2(ET/TC)-EYFP Cre AND NOT Flp Yes 137140 pAAV-nEF-Con/Foff 2.0-ChR2(ET/TC)-EYFP Cre AND Flp FRT...pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP Flp AND NOT Cre Yes 137142 pAAV-nEF-Con/Fon-ChR2-mCherry Cre AND Flp Yes ...2.0-ChR2-mCherry Cre AND NOT Flp FRT/F5 Yes 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry Flp AND NOT Cre Yes ...only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded to incorporate VCre in...Con/Foff hChR2(H134R)-EYFP Cre AND NOT Flp F3/F5 No 137163 pAAV-nEF-Con/Foff 2.0-ChR2-EYFP Cre AND NOT ...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon connection... the length of the fiber optic for the implant depends on the dorsal/ventral location of your region of... light-gated cation channel, Channelrhodopsin 2 (ChR2), exists in several states: closed/light receptive.... If you deliver a continuous stream of photons, Chr2 will open but will not be given an opportunity to...future. Now that your animals are all wired up and expressing your opsin of interest, it’s time to get to the... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

  6. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...shown that some channels, such as ChR2 and NpHR, can even be expressed simultaneously and still be individually...membrane when exposed to light. Neuronal firing depends on these charged particles and whether they make...will fire a signal. The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement... like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different subsets of spinal...spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that...

  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... a Drd1-Cre transgenic mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified... injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a Cre-dependent IL-1 beta...beta overexpression cassette. Expression of Cre in these neurons turns on overexpression of IL-1 beta. They...Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC projections to the NAc and decreased the locomotion... investigated the injection of AAV8 particles expressing an activating chemogentic receptor (hM3Dq) into...at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous...researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate...GFP-tagged Cre expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases... 0.4.Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression... EGFP, Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control...CAG tdTomato, Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth...Color-flipping switch. Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells...physically-separate mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases ...

  9. Brain Armamentarium

    Type
    Collection
    ...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755) Expression of ChR2(H134R)-EYFP in striatal...pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression in striatal ..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression in striatal ...pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression in striatal ...CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...

  10. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...opsins to operate at increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to...Invasiveness  Choosing the best set of tools therefore depends on what you are looking to study. Figure ...delivery is the same in both cases, but activation depends on small molecule activation (chemogenetics) or...optogenetic and chemogenetic tools can have their expression targeted to specific tissues, cell types or even...restricted to illuminated region Impacts all cells expressing receptors Invasiveness Mice require permanent...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    ...continual expression of both dCas9 and the gRNA. This expression can vary significantly depending on transgene...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...#140702) The pMAK461-64 plasmids were expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted syn-FLEX-axon-jYCaMP1s...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that exhibits...this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, ... the mCitrine gene. mCherry is constitutively expressed. Cells are then cultured and FACS sorted. Image...

  12. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...with an appropriate mouse line expressing a particular cre, viral expression of your opsin is no longer required...steps in the coming weeks. Expression of the optogenetic constructs Expression of your desired optogenetic...promoter-directed expression alone is extremely limited. Therefore, highly expressing, generic neuronal...opsin allows cell type specificity dependent on the promoter of the cre recombinase while still taking advantage...Finally, expression of the opsin in a mouse line will likely be less than that of viral expression but may...stimulation. Testing opsin expression  Regardless of the method of expression of your opsin gene, it is...Viruses that only express the fluorescent proteins are used as a critical control. Depending on the AAV serotype...

  13. Optogenetics Guide

    Type
    Guide
    ...line, such as for example the VGAT-ChR2 mouse, where ChR2 is expressed under the control of the vesicular...reinhardtii ChR2 Widely used light-gated cation channel from Chlamydomonas reinhardtii (CrChR2) 470 ChR2/H134...light activated cation channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance at physiological... C1C2 ChR1-ChR2 chimera 470 ChIEF, ChEF, ChD Engineered by chimeragenesis of ChR1 and ChR2 450-470 C1V1...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...aminobutyric acid (GABA) transporter (VGAT) and ChR2 is thus expressed in all GABAergic neurons. In this case,...delivery results in more localized expression of the opsin. Depending on the virus and promoter system ...

  14. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...strong opsin expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such...cells with an inactive opsin gene; in Cre expressing cells, however, Cre induces the recombination of the ...channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The...combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter. Once injected...not naturally light-sensitive, so the selective expression of opsin genes in targeted cells makes it possible...How to get an opsin into the brain In order to express opsins within the brain, researchers inject genetically...relatively stable extra-chromosomally long-term expression and low ability to induce innate immune responses...

  15. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...directs strong, ubiquitous expression. This is a Cre-dependent switch that expresses nuclear mCherry in the...the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors ...to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre...ubiquitous expression. pAAV-mDlx-NLS-mRuby2 (99130-AAVrg): The Dlx element directs expression in forebrain...see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory...pAAV-EF1a-fDIO-Cre (121675-AAV9, 121675-AAVrg)  pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1...entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and ...

  16. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2...only interpret their results, but also confirm expression in target cells. While the various AAV serotypes... can be used to achieve different patterns of expression, researchers often need to determine these transduction...calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options are also...under control of the CAG promoter for ubiquitous expression. (For more details on this technology, see our...Fon hChR2(H134R)-EYFP 26975 AAVrg pAAV-CaMKIIa-hChR2(H134R)-mCherry 26976 AAV5 pAAV-hSyn-hChR2(H134R...packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)...

  17. Immunology Research Plasmids and Resources

    Type
    Collection
    ...day. Their ability to protect against infection depends on two systems that work in concert: the innate...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...of activated T-cells, cytoplasmic, calcineurin-dependent 1 MGC138448, NF-ATC, NFAT2, NFATc NFATC2 nuclear...of activated T-cells, cytoplasmic, calcineurin-dependent 2 NFAT1, NFATP NFATC3 nuclear factor of activated...activated T-cells, cytoplasmic, calcineurin-dependent 3 NFAT4, NFATX NFATC4 nuclear factor of activated T-...
Showing: 1 - 17 of 17 results