We narrowed to 20 results for: Naga;
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TypeBlog Post...luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University have developed...signal. Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored...imaging. Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing...
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Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post.../10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016... -
Components of CRISPR/Cas9
TypeBlog Post...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...ACS Chem Biol. 2010 Feb 19;5(2):215-22. Takeharu Nagai ATP ATP sensor for Yeast (AT1.03 derived) Dynamic...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog Post...D, Serebrenik A, Shaban N, Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...kinetics and high signal/noise ratio. Here, Takeharu Nagai and colleagues present an improved genetically encoded... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...PMID: 29505611. PubMed Central PMCID: PMC5837189. Nagai, Takeharu, et al. "Expanded dynamic range of fluorescent... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post....2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...j.ymthe.2004.05.024 Dudek AM, Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH... -
Luciferase Plasmid Collection
TypeCollection...CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression... -
CRISPR History and Development for Genome Engineering
TypeCollection...Cell . 154(2):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed... -
CRISPR Guide
TypeCollection...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s... -
Chemogenetics Guide
TypeGuide.../doi.org/10.1126/science.aav5282 PMID: 30872534 Nagai, Y., Miyakawa, N., Takuwa, H., Hori, Y., Oyama, ... -
Adenovirus Guide
TypeGuide...) PMID: 17546019 (Link opens in a new window) Matsunaga, W., & Gotoh, A. (2023). Adenovirus as a vector... -
CRISPR Guide
TypeGuide...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s...