Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
Type
Blog Post
...single stranded regions with homology to anneal
a polymerase, to fill in the gaps
a DNA ligase, to seal the...than 200 nucleotides before the annealing and polymerization steps could occur. Secondly, it does not work...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...