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Showing: 1 - 9 of 9 results
  1. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...derived from Streptococcus pasteurianus  or the type II CRISPR3 module of Streptococcus thermophilus (the popular...popular Streptococcus pyogenes system does not work well in Caulobacter). To test their CRISPRi system...
  2. Doudna Lab CRISPR Plasmids

    Type
    Collection
    ...Cas9. 39314 pMJ824 : Streptococcus thermophilus Cas9. 39315 pMJ825 : Streptococcus pyogenes Cas9 D10A mutation...the links below: ID Plasmid 39312 pMJ806r : Streptococcus pyogenes Cas9. 39313 pMJ823 : Listeria innocua... mutation. 39316 pMJ826 : Streptococcus pyogenes Cas9 H840A. 39317 pMJ839 : Neisseria meningitidis Cas9...Cas9. 39318 pMJ841 : Streptococcus pyogenes Cas9 D10A, H840A. 41091 pHMGWA-Pa14Csy4 : Pseudomonas aeruginosa...
  3. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Neisseria meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema denticola... Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E...
  4. Church Lab CRISPR Plasmids

    Type
    Collection
    ...nuclease and a transcriptional activator. Streptococcus thermophilus #1 (ST1): Almost as small as NM, ST1 ... in bacteria. Cas9 proteins in human cells: Streptococcus pyogenes (SP): This is the classical Cas9 protein...Plasmid Description 48669 M-ST1cas Mammalian S. thermophilus #1 Cas9 expression, human optimized 48670 M-...cloDF13/spectinomycin 48647 DS-ST1cas Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13...
  5. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...include the use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated Cas9...infection of certain tissues. However, packaging Streptococcus pyogenes (SpCas9) and a gRNA together (~4.2 ...
  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola...below.  Cas9 species PAM sequence ’) Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus...
  7. CRISPR Guide

    Type
    Guide
    ...Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (...Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...
  8. CRISPR Guide

    Type
    Collection
    ...Neisseria meningitidis (NM) 3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (...Sequences Species/Variant of Cas9 PAM Sequence* Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...
Showing: 1 - 9 of 9 results