...-AAV9 pAAV-Ef1a-DIO-PPO-Venus AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven...

...the acceptor fluorophore, a circularly permuted Venus. In the case of pHlash, the signal from the fluorophore...host-tropism. These tools open up many powerful avenues through which one can examine molecular events ...

...as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit... are able to fulfill our mission by generating revenue through distribution of research materials. Our..." is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution... nonprofits should have some level of positive revenue to build reserves so the company is sustainable...The difference is what nonprofits do with their revenue. A nonprofit organization cannot distribute profits...

... For example, the APT1-mVenus fusion localizes at the Golgi, whereas the mVenus-APT1 fusion does not (... (figure 2). In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded...therefore this protein is mislocalized. The APT1-mVenus fusion is clearly a better choice to work with....

...Bacterial Expression mVenus 515 528 53 6 Monomer (A206K) mVenus N1 - Mammalian Expression mVenus C1 - Mammalian...Expression mVenus-pBAD - Bacterial Expression moxVenus 514 526 44 Monomer (A206K) moxVenus - Mammalian...

... fused to mVenus linked to a mitochondrial localization sequence TOM20 (pTriEX Tom20 mVenus BoPD). In ...

...expressed a fusion of keratin with mGold (top) and mVenus (bottom). Cells were imaged every 30 seconds for... up to 5-fold more photostable and as bright to mVenus, the parent YFP, and is the most photostable YFP...

...RNA without tagging,” she said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic...

...not make changes to the genome, thus providing an avenue for temporary/reversible CRISPR modification. In...

...silencer. The current work also proposes promising avenues for future engineering to shift the spectra and...

...adult. My initial research directions followed that avenue with projects studying developmentally programmed...

...enabled scientists to pursue creative research avenues previously unavailable to them. With these fantastic...

... Abitua and the other organizers found several avenues to keep the audience engaged. They first sent a...

... The plasmid for co-expressing mTurquoise2 with mVenus as a reference protein is available from Addgene...

...will improve the utility of CRISPR/Cas9 in new avenues of research. The use of multiple gRNAs targeting...

...causes of some human conditions, opening up new avenues for therapeutics that treat disease at the molecular...

...graduate students will pursue this newly available avenue. Interestingly, both of these changes share a common...

...various professionals, and there were multiple avenues for professional development including workshops...

...initiatives, like Addgene’s Data Hub, provide an avenue to more easily solve these frustrating and often...

...Super-resolution microscopy: a brief history and new avenues. Philosophical Transactions Royal Soc 380:20210110...