...position.  Check out Joanne's Reddit AMA Here are 6 steps to submitting a resume that will give you a ...letter the first page of your resume pdf document. 6) Use your network! During the entire process, use ... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013...Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055. Epub 2014 Oct 19. PubMed ....Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

...mCherry nanobody. Only insertion into loops 1 and 6 resulted in light controlled binding, but with opposite...into loop 1 caused dark-induced binding while loop 6 insertion caused blue-light induced binding. The lab...with dark-induced binding, but insertion into loop 6 generated both blue light inducible and dark-inducible...Loop 1 dark-induced dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced...Addgene blog Learn about using nanobodies to control protein activity Read about RANbodies, reporter ...reporter nanobody fusions Learn more about using the secondary nanobody toolbox for immunodetection Resources...Find nanobody expression plasmids here Learn more about optogenetics in Addgene's optogenetics guide ...

...and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial host strain for...E17TAG GFP has been achieved with the Rinehart system (6); however different proteins will likely achieve different...context-specific effects of UAG suppression, as described (6). To determine the stoichiometry of phospho-incorporated...expression vector used for phosphoserine incorporation (6). This will give the researcher both a phosphoserine...333.6046 (2011): 1151-1154. PubMed PMID: 21868676. 6. Pirman, Natasha L., et al. "A flexible codon in genomically...programmable protein phosphorylation." Nature communications 6 (2015). PubMed PMID: 26350500. PubMed Central PMCID... one of the MEK1 phosphomimetic residues in the above study with phosphoserine (to create a pSer/phosphomimetic...

...positions available in academia (5, 6). Academic data and the labor market for PhDs Later this week, postdocs...number of graduate students accepted in STEM fields (6). Melanie Sinche is a STEM career counselor, Director...science Master’s programs. This will be the one of 6 great panels at the Future of Research Symposium Boston...PMID: 24137543. Pubmed Central PMCID: PMC3787295. 6. Marder, Eve. "Looking out for future scientists."... the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the Labor and Worklife...increasing data collection about the scientific workforce, in particular about postdocs. Among other involvements...Research Symposium Boston 2015 – Academic Data and the Labor Market. For more information on the symposium as...

...polyhistidine tag? The polyhistidine tag is a string of 6 or more histidine residues expressed in frame on the...string of histidines in the tag is typically between 6 to 9 residues long. The small size of the polyhistidine...Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind...virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling...that this blog has answered all of your questions about polyhistidine tags. For more antibody-related information...

...526–534. https:/doi.org/10.1016/S1525-0016(03)00023-6. PMID: 12727116. Borkenhagen, L. K., Fieldhouse, J...Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https://doi.org/10.1371/journal.ppat.1004821...adenoviruses. J Clin Pathol Suppl (R Coll Pathol)., 6, 39. https://doi.org/10.1136/jcp.s3-6.1.39. PMID: ...implications for their use as vaccines. Blood, 110(6), 1916–1923. https://doi.org/10.1182/BLOOD-2007-02...Vaccination and Cancer Gene Therapy. Curr Gene Ther, 13(6), 421–433. https://doi.org/10.2174/1566523213666131125095046...this introduction gives a sense of what AdVs are about – and we hope that it inspires further use of existing...

... column. They can be pricey, but well worth it. 6. Edit your DNA sequence Finally, when you do see a...and troubleshooting your DNA sequencing results Above (top right corner of this post) is an example of...

...hustle on the side. “I actually stumbled upon, about 6 months in, this opportunity to do scientific editing...” But Caitlin’s persistence paid off: “I spent about 6 months just editing and searching for a job and... day and editing at night. It was not the easiest 6 months. But it gave me the foundation to be a much...Science Communication blog series, you’ll learn about Caitlin Runne-Janczy, a Product Development Manager...G-proteins at the time. This program takes what you know about it [your area of research], and teaches you how ...Additional Resources on the Addgene Blog Learn more about alternative science careers here Be like Caitlin...just for good measure, here’s a few other posts about developing transferable skills while in grad school...

...Nature Methods. 2011 Mar;8(3):260-6. doi: 10.1038/nmeth.1567. Epub 2011 Feb 6. PubMed. Improved tools for the...Methods. 2013 May 5:10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. PubMed. Zebrabow: multispectral cell...Sanes JR. Nature Reviews Neuroscience. 2008 Jun;9(6):417-22. doi: 10.1038/nrn2391. Epub 2008 Apr 30. PubMed...(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011 Feb 6. PubMed. Flybow: genetic multicolor cell labeling ...integration of multiple copies of the construct (about 8 in Brainbow-1.0 mice.) The combinatorial effect...with the goal of overcoming the limitations listed above. First, they screened a variety of fluorescent proteins...method has become very useful in lineage tracing; “Zebrabow” was used to trace the development of the corneal...

...Your chosen restriction site for cloning (usually 6-8bp) Hybridization Sequence: The region of the primer...more information). Thus, we recommend that you add 3-6 bases upstream of your restriction site to improve...improve cutting efficiency. You can generally add any 6 bases, but you should ensure that the bases do not ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time...Additional Resources on the Addgene Blog Learn About Other Cloning Techniques Catch Up on All Things ...

...development of genetic targets and RNA therapies. [6]. In 2014, we wrote a blog post on CRISPR’s potential... also possible through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene...Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6. Wu et al. 2020. . “Advances in CRISPR/Cas-based Gene...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

...finishing my MSc. The masters program was 6 months of classes and 6 months of hands-on lab work for a dissertation...I started  looking for other career options after 6 months. Returning to university to study a Masters...VISA, which means I can gain employment for up to 6 months in Australia or another country of the Commonwealth...University of Queensland required a scientist for a 6 month project to validate some genomic changes, so...really passionate about during my degree. I loved parasitology. However, when I enquired about roles in this...with an overview of my experience and how I felt about the different positions to enable them to decide...

...corresponds to the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid...recombination of plasmids. Nature. 1981 Nov 12;294(5837):184-6. doi: 10.1038/294184a0. PMID: 7029308. Goebel W, Helinski...catastrophe hypothesis. Mol Microbiol. 1993 Jun;8(6):1031-8. doi: 10.1111/j.1365-2958.1993.tb01648.x. ...Apr;36(4):1097-103. doi: 10.1016/0092-8674(84)90060-6. PMID: 6323019. Williams JA, Carnes AE, Hodgson CP... processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002/bit.22347. PMID: 19408315; PMCID...colonies using one of the detection methods described above can help ensure the isolation of the monomeric version...plasmid DNA production platform incorporating low metabolic burden seed-stock and fed-batch fermentation processes...

... ThermoFisher Scientific and can be used for only 6-8 reprogramming reactions. Another drawback of using...virus is the persistence of residual viral material [6]. The complete removal of the residual viral particles...Protoc, 2012. 7(4): p. 718-28. PubMed PMID: 22422317. 6. Diecke, S., et al., Recent technological updates ...non-integrating episomal vectors. PLoS One, 2011. 6(11): p. e27956. PubMed PMID: 22132178. PubMed Central...successfully to derive iPSCs from PBMCs. Most laboratories use Sendai virus to reprogram PBMCs efficiently...establishment of virus-free hiPSCs usually require about 5-10 passages. Furthermore, working with Sendai ...source of somatic cells. This translates to only about 16 hiPSC colonies per 100 ml of starting blood sample...

...provirus. Sci. Rep. 6, 20064 (2016). PubMed PMID: 26829887. PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al...detection limits of about 5% mutant targets have been reported for mismatch nuclease assays (2, 6, 7). Ability...advantages over mismatch nuclease assays that will be elaborated below. Digital PCR Digital PCR (dPCR) involves...

...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed...characterizing the 4D nucleome. CRISPRainbow: Scaling up to 6 colors + white Color Hairpin Blue MS2-MS2 Green...Additional Resources on the Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the...

...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

...downstream of the highly conserved glutamine-fructose-6-phosphate aminotransferase (glmS) gene in the recipient...each of the 40 library strains was measured after 6 and 12 generations with or without induction of CRISPRi...fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi can...Addgene blog Learn about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko...

...Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–313. https://doi.org/10.1159/000490898 Gibbs...J. (2009). Rats! Disease Models & Mechanisms, 2(5–6), 206–210. https://doi.org/10.1242/dmm.002733 Idalia...Arabidopsis as a model plant. The Plant Journal, 61(6), 909–921. https://doi.org/10.1111/j.1365-313x.2009.04086...and Aging Research: A Mini-Review. Gerontology, 57(6), 565–571. https://doi.org/10.1159/000323761 Modlinska... more about model organisms in the first Five Popular Model Organisms blog post Read more about plasmids...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...