...Phy–PIF Light-controlled Phytochrome Interacting Factor 6 (PIF6) to PhyB (Phy) interaction 660 Cryptochromes...targeted brain circuits. J Neurosci. 26(41):10380-6. PMID 17035522 Dugué GP, Akemann W, Knöpfel T. A comprehensive...transgene expression in neurons. Front Neural Circuits. 6:47. PMID 22866029 Wietek J, Beltramo R, Scanziani ...control derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

...libraries. First, each library typically contains ∼3-6 gRNAs per gene to ensure modification of every target...Davidson AR, Maxwell KL, Doudna JA. Cell . Sep 7;170(6):1224-1233.e15. PMID: 28844692 Engineered Cpf1 variants... Wang Z, Kinney JB, Vakoc CR. Nat Biotechnol . 33(6):661-7. PMID: 25961408 Electroporation enables the...Qi LS, Kampmann M, Weissman JS. Cell . 159(3):647-6. PMID: 25307932 Improving CRISPR-Cas nuclease specificity... Fujii H. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Genome engineering using the CRISPR-Cas9..., Joung JK, Sander JD. Nat Biotechnol . 31(9):822-6. PMID: 23792628 Multiplex genome engineering using..., Norville JE, Church GM. Science . 339(6121):823-6. PMID: 23287722...

...Exclusively Activated by Designer Drugs. ACS Chem Neurosci 6:476–484. PMID: 25587888 Coward P, Wada HG, Falk MS...receptor-effector complex for gene therapy. J Biol Chem. 276:1596-6. PMID: 11402033 Sternson SM, Roth BL (2014). Chemogenetic.... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...tissue-specific Cre is provided. Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors...

...alpha-toxin , and resistance to to the nucleotide analog 6-thioguanine . Next-generation sequencing is the only... to conduct a pooled library screen. The figure above is an outline of how a gRNA library is used. Below...simultaneously in a single reaction. As discussed above, access to this technology is crucial for conducting...

...Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting sanger sequencing...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...codon. Below you will find helpful resource tables about the genetic code. This table includes the nucleotide...

...protein location in a cell or tissue sample. Figure 6: Immunofluorescence, in which fluorophore-conjugated...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow Cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...