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Showing: 1 - 20 of 21 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~6 18,343 Human ...contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-...134582 134583 134584 Knockout Fly Perrimon 3rd 8 6 6 7,956 17,827 59,406 Dual sgRNA CRISPRi Libraries ...Superti-Furga 3rd 6 3,342 Human SLC Knockout Library 132552 Knockout Human Superti-Furga 3rd 6 2,609 Human ...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...immunoglobulin heavy diversity 6-25 IGHD625 IGHD6-6 immunoglobulin heavy diversity 6-6 D(N4), IGHD66 IGHD7-27 ...beta variable 6-5 TCRBV13S1, TCRBV6S5, TRBV65 TRBV6-6 T cell receptor beta variable 6-6 TCRBV13S6A2T, ...IL6 interleukin 6 (interferon, beta 2) BSF2, HGF, HSF, IFNB2, IL-6 IL6R interleukin 6 receptor CD126, ...immunoglobulin heavy diversity 6-13 DN1, IGHD613 IGHD6-19 immunoglobulin heavy diversity 6-19 IGHD619 IGHD6-25 immunoglobulin...MGC22745, MGC32715, MGC88810, V1 IGKV1-6 immunoglobulin kappa variable 1-6 IGKV16, L11 IGKV1-8 immunoglobulin...chemokine (C-C motif) receptor 6 BN-1, CD196, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2):161-6. Gary Yellen ATP/ADP Fluorescent sensor of ATP...Indicator. Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. Edward Boyden Calcium GCaMP6f/m/s calcium sensors...dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924. Yubin Zhou Calcium Twitch calcium indicator...intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn2+ FRET ...Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding...range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red ...Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. Jin Zhang cAMP (cyclic AMP) Yellow-emitting homoFRET...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...included. Ensure there are 2 x 10 6 cells per CRISPR pair. Resuspend 2 x 10 6 cells in 100 μl of electroporation...half for screening and primer validation (see step 6 ). NOTE: This protocol is for suspension cells. Adherent...thermocycler and run the following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the ...from clones. Run sample in thermocycler: 65 °C for 6 min and 98 °C for 2 min to extract gDNA. Screen each... conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion...

  5. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... Venus fluorescent proteins connected via a 5 and 6 amino acid linker, respectively VVVV Anisotropy/brightness...Venus fluorescent proteins connected via a 5, 5 and 6 amino acid linker, respectively VVVVV Anisotropy/brightness...standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats pET28CLY7 Peptide linker standard consisting...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

  6. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...Trabecular Meshwork in Mouse and Rat. PLoS One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction...Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666 AAV44.9(E531D) When using...Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666 Don’t See What You’re Looking...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

  7. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...domain by author. Source: Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class...NR61, RTR, hGCNF, hRTR nuclear receptor subfamily 6, group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

  8. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Pan-QKI scFv [N147/6] N147/6 scFv Pan-QKI Human Mouse 211606 QKI-6 scFv [N182/17] N182/17 QKI-6 Human Mouse ...190543 Shank2 scFv [N23B/6] N23B/6 scFv Shank2 Rat Mouse 190544 Synaptotagmin-6 scFv [N270/47] N270/47 ...] S100A5 Rat Mouse IgG2a 114561 Anti-QKI-6 [N182/17.1R] QKI-6 Human Mouse IgG2a 114562 Anti-SynCAM4 [N244...Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK [...channel Mouse Mouse IgG2b 206661 Anti-QKI-6 [N182/17R-2b] QKI-6 Human Mouse IgG2b 206662 Anti-PARIS/ZNF746... Rat Mouse 182094 Nav1.7 Na+ channel scFv [N68/6] N68/6 Nav1.7 Na+ channel Human Mouse 182095 GFP scFv... Mouse 206733 Kv7.4/KCNQ4 K+ channel scFv [N43/6] N43/6 scFv Kv7.4/KCNQ4 K+ channel Human Mouse 206734...

  9. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...iSPI_pSer_Subpool#5 Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library...FJ, and Rinehart J. Nature Communications . 2015. 6, 8130. PubMed Robust Production of Recombinant Phosphoproteins...Rinehart J, and Jewett MC. Nature Communications . 2015. 6, 8168. PubMed The PINK1-PARKIN Mitochondrial Ubiquitylation...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

  10. Rett Syndrome

    Type
    Collection
    ...development through the first 6 months of age, followed by developmental delay between 6 and 18 months of age....Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion of Xist Unpublished... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

  11. Brain Initiative Collection

    Type
    Collection
    ...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99133-AAV1 pAAV-CAG-fDIO-mNeonGreen...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

  12. Adenovirus Plasmids

    Type
    Collection
    ...for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion of GFP expression...information about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus...

  13. AAVED

    Type
    Collection
    .... Tentative Meeting Agenda Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with ...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

  14. CRISPR Guide

    Type
    Collection
    ...libraries. First, each library typically contains ∼3-6 gRNAs per gene to ensure modification of every target...Davidson AR, Maxwell KL, Doudna JA. Cell . Sep 7;170(6):1224-1233.e15. PMID: 28844692 Engineered Cpf1 variants... Wang Z, Kinney JB, Vakoc CR. Nat Biotechnol . 33(6):661-7. PMID: 25961408 Electroporation enables the...Qi LS, Kampmann M, Weissman JS. Cell . 159(3):647-6. PMID: 25307932 Improving CRISPR-Cas nuclease specificity... Fujii H. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Genome engineering using the CRISPR-Cas9..., Joung JK, Sander JD. Nat Biotechnol . 31(9):822-6. PMID: 23792628 Multiplex genome engineering using..., Norville JE, Church GM. Science . 339(6121):823-6. PMID: 23287722...

  15. Viral Vectors

    Type
    Collection
    ... cancer gene therapy. Curr Gene Ther. 2013 Dec;13(6):421-33. doi: 10.2174/1566523213666131125095046. PMID...Virus Should I Use? Use this guide to learn more about the different types of viruses that are commonly...temporary in the host. Use this guide to learn more about the different types of viruses that are commonly...found in the guides for each virus type, linked above. Virus Expression Genome Packaging Capacity Virus...

  16. TALEN Guide

    Type
    Collection
    ...AJ, Voytas DF. Science 2011 Sep 30;333(6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of...researchers who are learning to wield them. Learn more about Addgene’s TAL effector and TALEN kits here . References...

  17. Zhang Lab CRISPR Page

    Type
    Collection
    ...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013...Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6. doi: 10.1038/nbt.3055. Epub 2014 Oct 19. PubMed ....Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids...

  18. COVID-19 Resources

    Type
    Collection
    ...hACE2, potentially increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in ...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...to distribute SARS-CoV-2 plasmids. To read more about this partnership and the plasmids that are available...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

  19. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...01015-5 As for target selection, we usually pick 3-6 guides around the region to find the most efficient... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology. Genome..., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

  20. Cre-lox system

    Type
    Collection
    ...Transgenic Mice. Proc Natl Acad Sci. Jul 15;89(14):6232-6. PubMed . Matsuda T, Cepko CL. 2007. Controlled Expression...10380-5. doi:10.1073/pnas.0403954101. Epub 2004 Jul 6. PubMed . Do you have suggestions for other plasmids...Learn about the Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use...
Showing: 1 - 20 of 21 results