...Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~6 18,343 Human ...contained in the library represent all 4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-...134582 134583 134584 Knockout Fly Perrimon 3rd 8 6 6 7,956 17,827 59,406 Dual sgRNA CRISPRi Libraries ...Superti-Furga 3rd 6 3,342 Human SLC Knockout Library 132552 Knockout Human Superti-Furga 3rd 6 2,609 Human ...

...immunoglobulin heavy diversity 6-25 IGHD625 IGHD6-6 immunoglobulin heavy diversity 6-6 D(N4), IGHD66 IGHD7-27 ...beta variable 6-5 TCRBV13S1, TCRBV6S5, TRBV65 TRBV6-6 T cell receptor beta variable 6-6 TCRBV13S6A2T, ...IL6 interleukin 6 (interferon, beta 2) BSF2, HGF, HSF, IFNB2, IL-6 IL6R interleukin 6 receptor CD126, ...immunoglobulin heavy diversity 6-13 DN1, IGHD613 IGHD6-19 immunoglobulin heavy diversity 6-19 IGHD619 IGHD6-25 immunoglobulin...MGC22745, MGC32715, MGC88810, V1 IGKV1-6 immunoglobulin kappa variable 1-6 IGKV16, L11 IGKV1-8 immunoglobulin...chemokine (C-C motif) receptor 6 BN-1, CD196, CKR-L3, CKR6, CKRL3, CMKBR6, DCR2, DRY-6, GPR-CY4, GPR29, GPRCY4...

...reporter of ATP:ADP ratio. Nat Methods. 2009 Feb . 6(2):161-6. Gary Yellen ATP/ADP Fluorescent sensor of ATP...Indicator. Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. Edward Boyden Calcium GCaMP6f/m/s calcium sensors...dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924. Yubin Zhou Calcium Twitch calcium indicator...intracellular Zn(2+) homeostasis. Nat Methods. 2009 Oct;6(10):737-40. Maarten Merkx Zinc eZinCh-2 Zn2+ FRET ...Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding...range for dual-color imaging. PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red ...Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. Jin Zhang cAMP (cyclic AMP) Yellow-emitting homoFRET...

...included. Ensure there are 2 x 10 6 cells per CRISPR pair. Resuspend 2 x 10 6 cells in 100 μl of electroporation...half for screening and primer validation (see step 6 ). NOTE: This protocol is for suspension cells. Adherent...thermocycler and run the following program: 65 °C for 6 min, 98 °C for 2 min to extract gDNA. Measure the ...from clones. Run sample in thermocycler: 65 °C for 6 min and 98 °C for 2 min to extract gDNA. Screen each... conditions optimized on the bulk cells (see step 6 ). Select the clones identified with the desired deletion...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion...

... Venus fluorescent proteins connected via a 5 and 6 amino acid linker, respectively VVVV Anisotropy/brightness...Venus fluorescent proteins connected via a 5, 5 and 6 amino acid linker, respectively VVVVV Anisotropy/brightness...standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats pET28CLY7 Peptide linker standard consisting...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins ...

...Trabecular Meshwork in Mouse and Rat. PLoS One . Jun 8;10(6). PMID: 26052939 Lu, et al. 2016. AAV-mediated transduction...Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666 AAV44.9(E531D) When using...Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID: 32304666 Don’t See What You’re Looking...Information The plasmid below is available in each of the above serotypes and buffers. Please click on the plasmid...

...domain by author. Source: Wikipedia, accessed November 6, 2013. Author: Boghog2 Nuclear receptors are a class...NR61, RTR, hGCNF, hRTR nuclear receptor subfamily 6, group A, member 1 PGR Plasmids PGR, NR3C3, PR progesterone...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

...Pan-QKI scFv [N147/6] N147/6 scFv Pan-QKI Human Mouse 211606 QKI-6 scFv [N182/17] N182/17 QKI-6 Human Mouse ...190543 Shank2 scFv [N23B/6] N23B/6 scFv Shank2 Rat Mouse 190544 Synaptotagmin-6 scFv [N270/47] N270/47 ...] S100A5 Rat Mouse IgG2a 114561 Anti-QKI-6 [N182/17.1R] QKI-6 Human Mouse IgG2a 114562 Anti-SynCAM4 [N244...Mouse IgG2a 188216 Anti-Synaptotagmin-6 [N270/47R] Synaptotagmin-6 Mouse Mouse IgG2a 188217 Anti-CASK [...channel Mouse Mouse IgG2b 206661 Anti-QKI-6 [N182/17R-2b] QKI-6 Human Mouse IgG2b 206662 Anti-PARIS/ZNF746... Rat Mouse 182094 Nav1.7 Na+ channel scFv [N68/6] N68/6 Nav1.7 Na+ channel Human Mouse 182095 GFP scFv... Mouse 206733 Kv7.4/KCNQ4 K+ channel scFv [N43/6] N43/6 scFv Kv7.4/KCNQ4 K+ channel Human Mouse 206734...

...iSPI_pSer_Subpool#5 Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library 188532 iSPI_pSer_Subpool#7 Pooled Library...FJ, and Rinehart J. Nature Communications . 2015. 6, 8130. PubMed Robust Production of Recombinant Phosphoproteins...Rinehart J, and Jewett MC. Nature Communications . 2015. 6, 8168. PubMed The PINK1-PARKIN Mitochondrial Ubiquitylation...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

...development through the first 6 months of age, followed by developmental delay between 6 and 18 months of age....Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse line with conditional deletion of Xist Unpublished... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAV9 pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99130-AAVrg pAAV-mDlx-NLS-mRuby2...expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer Viviana Gradinaru 99133-AAV1 pAAV-CAG-fDIO-mNeonGreen...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

...Novophage to Sample 6. To learn more about Novophage's successful transformation into Sample 6 and Dr. Koeris...25 : Introduction 1:25 - 6:19 : Dr. Koeris' graduate work in the Collins' lab 6:19 - 8:26 : Dr. Koeris'...food products prior to Sample 6 16:27 - 18:02 : Benefits of the Sample 6 technology and how It works 18...56 : Why Sample 6's first test was for Lysteria 19:56 - 24:41 : Who is using Sample 6 and why? 24:41 -...Visit the Sample 6 website Check out Dr. Koeris' Website, biotechstart.org Read Sample 6's Paper on Bacteriophage-based...use in a biotech startup 12:16 - 15:15 : Why Sample 6 began to develop tests for food products as opposed...Directors. We're excited to watch both Addgene and Sample 6 grow with his help! Special thanks to our sound engineer...

...productive with the granting agency’s investment. 6. Failure is part of the process, keep your head up...introduction to my area, a line about the research I will be doing, and a line about what I hope to discover....themselves many times and will have useful advice about what reviewers in your area are looking for. Remember...One way to add originality to your work is to collaborate with specialists in different fields that can...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...outcomes can include poster presentations, talks about your work, or even the successful use of your preliminary...quality application. Even following this advice, only about 50% of my grant applications have been successful...

...for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion of GFP expression...information about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus...

... tweeted 6 times: #fail. I’d hoped to use Twitter professionally to network, learn more about alternative... Figure 3: Top 20 Domains Tweeted by Scientists. 6. The top scientific domains tweeted by biologists ...fields than scientists outside their fields Figure 6: Follower Network of Scientists on Twitter. Each dot...Check out this post. Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking for Scientists....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

.... Tentative Meeting Agenda Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with ...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

...libraries. First, each library typically contains ∼3-6 gRNAs per gene to ensure modification of every target...Davidson AR, Maxwell KL, Doudna JA. Cell . Sep 7;170(6):1224-1233.e15. PMID: 28844692 Engineered Cpf1 variants... Wang Z, Kinney JB, Vakoc CR. Nat Biotechnol . 33(6):661-7. PMID: 25961408 Electroporation enables the...Qi LS, Kampmann M, Weissman JS. Cell . 159(3):647-6. PMID: 25307932 Improving CRISPR-Cas nuclease specificity... Fujii H. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Genome engineering using the CRISPR-Cas9..., Joung JK, Sander JD. Nat Biotechnol . 31(9):822-6. PMID: 23792628 Multiplex genome engineering using..., Norville JE, Church GM. Science . 339(6121):823-6. PMID: 23287722...

...-Integrin alpha V beta 6 [IPI-ITGAV/ITGB6.2] Integrin alpha-V,Integrin beta-6 Human, Mouse IgG1 Rabbit...-Integrin alpha V beta 6 [IPI-ITGAV/ITGB6.3] Integrin alpha-V,Integrin beta-6 Human, Mouse IgG1 Rabbit...-Integrin alpha V beta 6 [IPI-ITGAV/ITGB6.4] Integrin alpha-V,Integrin beta-6 Human, Mouse IgG1 Rabbit...-Integrin alpha V beta 6 [IPI-ITGAV/ITGB6.12] Integrin alpha-V,Integrin beta-6 Human, Mouse IgG1 Rabbit...roles of individual integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the...recombinant antibodies and plasmids. To encourage collaboration and education in protein science, IPI hosts ...events and training courses on technologies and laboratory techniques related to antibody discovery. You...

...position.  Check out Joanne's Reddit AMA Here are 6 steps to submitting a resume that will give you a ...letter the first page of your resume pdf document. 6) Use your network! During the entire process, use ... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

... cancer gene therapy. Curr Gene Ther. 2013 Dec;13(6):421-33. doi: 10.2174/1566523213666131125095046. PMID...Virus Should I Use? Use this guide to learn more about the different types of viruses that are commonly...temporary in the host. Use this guide to learn more about the different types of viruses that are commonly...found in the guides for each virus type, linked above. Virus Expression Genome Packaging Capacity Virus...