... of well-annotated deletion strains is available (6). Methionine synthesis in S. cerevisiae (Figure 1)...mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1 plasmid...PubMed PMID: 9409150. PubMed Central PMCID PMC232622. 6. Winzeler, D.A., Shoemaker, D.D., Astromoff, A., Liang...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...

...implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...wireless optogenetics. Science. 2013;340(6129):211-6. doi: 10.1126/science.1232437. PubMed PMID: 23580530...10.1016/j.neures.2011.01.007. PubMed PMID: 21238511. 6. Jeong, Jae-Woong, et al. Wireless Optofluidic Systems...distance so ideal implant placement should be about 0.4-0.5mm above the site of your stimulation. The scatter...scatters a great deal once it enters tissue, placement above the site of stimulation guarantees that the majority...instead). Laser stimulation         In the video above, Channelrhodopsin 2 (Chr2) has been expressed in...These lasers are relatively cheap and will run you about $2-3,000/laser. However, an important caveat is ...

...Secretion Libraries.” ACS Synthetic Biology, vol. 6, no. 6, American Chemical Society (ACS), 15 Mar. 2017...Constructs.” PLoS ONE, edited by Jean Peccoud, vol. 6, no. 2, Public Library of Science (PLoS), 18 Feb. ...vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp. 3393–405. Crossref, https://doi.org... Expression Vectors.” ACS Synthetic Biology, vol. 6, no. 7, American Chemical Society (ACS), 24 Apr. 2017... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila. ...

..., AAV vectors AAV1, AAV-2, AAV1-AAV2 hybrids, AAV-6, AAV-7, AAV-8, AAV-9 and AAVrh10 have been used in...Neuroscience 20.8 (2017): 1172-1179. PubMed PMID: 28671695. 6. Münch, Robert C., et al. "Off-target-free gene delivery...receptor-targeted viral vectors." Nature communications 6 (2015): ncomms7246. PubMed PMID: 25665714. Additional...Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control Process...viral infections. She is particularly passionate about HIV cure research and science communication.     ...

...mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA...putative operator sequences (CuO) (linked in groups of 6), allowing transcription to proceed. When cumate is...immediate early gene expression. Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101/gad.2.6.718. PMID: 2843425.Takara...can be used in the repressible manner described above, as well as the inducible manner detailed in our...necessary to have all 3 of the different binary systems above if they all behave very similarly?” The simple answer... at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not affect ...

... 38, pp. 14492–6, Sep. 2008. PubMed PMID: 18787116. PubMed Central PMCID: PMC2567143. 6. S. Radecke, F...single-stranded oligodeoxynucleotide (ssODN) as the donor [6]. The problem with this approach is that, since there.... As a gene editing enthusiast, he has spent over 6 years in the lab developing and using a variety of...transposase itself, please read on to learn more about this exciting technology from the folks at Transposagen...Additional Resources on the Addgene Blog Learn about the Sleeping Beauty Transposase Read up on Genome...

...single sgRNA outperformed another CRISPR library with 6 sgRNAS, highlighting the benefit of sgRNA design in...rules and  potential on- and off-target effects. The 6 best sgRNAs were selected per gene and divided into...knockout genetic screens." bioRxiv (2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system...Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled...large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources on...libraries for the mouse genome! Other Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...

...can be missed by single-pass two-hybrid screening (6). See the Tips section for more on how to increase...independent protein interaction detection methods (6). In order to bind DNA and activate a reporter gene... Chem. 2001; 73:2516-2521. Pubmed PMID: 11403293. 6. Chen, Y.C., Rajagopala S.V., Stellberger T., Uetz... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...

...deaths worldwide (statistics provided by Google on 6/4/20). Many countries entered lockdown for weeks to...The dataset includes 11,426 plasmid requests over 6 years for 35 papers (data was provided by Addgene ...were somehow imposed on scientists. Figure 6: Model fit. Orange line depicts observed data. Blue...in his laboratory. Learn more here. Additional resources on the Addgene blog Learn more about plasmid...

...recognition sites, which contain CpG dinucleotides in their 6-mer sequences, are rarely found in the transcriptomes...several hundred kilobases. Nature methods. 2009. May;6(5):343–345. Pubmed PMID: 19363495. 4. Josse J, Kaiser...PMID: 18410688. PubMed Central PMCID: PMC2373286. 6. Sladitschek HL, Neveu PA. MXS-Chaining: A Highly ...Resources on the Addgene Blog Read about Gibson Assembly Learn About Other Plasmid Cloning Techniques Plasmids...

...Nature communications 6 (2015). Pubmed PMID: 26158227. Pubmed Central PMCID: PMC4499870. 6. JAIN, R., et al...FP-fusion proteins. Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences of...moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly monomeric...Medicine. Her doctoral research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent...

...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (...stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on several other ...LSFM. In LSFM the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample...

...and cell types used for packaging viral particles (6), can influence vector performance. Open science initiatives...Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman...AAV9) Preparations. Human Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik...asked why they don’t share, many express concern about sharing negative data they cannot explain, perhaps...

... genome replication and viral gene transcription (6). The EBOV genome contains seven viral genes, each... ng L, and 2 μg minigenome plasmid per well (in a 6 well plate format). However, since plasmid quality...PMID: 24093048. PubMed Central PMCID: PMC3787895. 6. Weik, Michael, et al. "The Ebola virus genomic replication...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

... Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two different brain regions.  Image modified...Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLOS ONE, 8(9), e76310...Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Dudek...Article 5. https://doi.org/10.1038/s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

...cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...hACE2, potentially increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell protein 4) is involved in ...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...to distribute SARS-CoV-2 plasmids. To read more about this partnership and the plasmids that are available...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

... (2022). https://doi.org/10.1038/s41593-022-01094-6. (This work was also shared as a preprint: https:/...proto-oncogene product. Mol Cell Biol. Dec;5(12):3610-6.(1985). doi: 10.1128/mcb.5.12.3610-3616.       ...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...

... Barcodes consist of an 8bp variable region and a 6 bp fixed region. The variable region acts as a unique... transcriptional states were seen as early as day 6. All together these results suggest that iEP reprogramming...Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS) and reprogramming...

...library construction, and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase ...junction in 16 wells of a 96-well plate, allowing 6 conditions to be screened per plate. The results indicated...PubMed PMID: 1986365. PubMed Central PMCID: PMC50775. 6. Barany, Francis, and David H. Gelfand. "Cloning, ... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...