Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
Type
Blog Post
...a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin (Ace2N) with the red ...Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet...seven or eight amino acid linker between them. The Ace-mScarlet voltage indicator works like many FRET-opsin... an increase in the neuron’s membrane potential, Ace absorbance increases and results in higher quenching...blue-light-sensitive channelrhodopsins.
Find the Ace-mScarlet voltage indicator plasmids at Addgene!
Beck...mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image from Beck and Gong, 2019.
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