...editing portion of the protein, they examined ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA... functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA... editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also adjusted the structure...RNA. Using an ADAR2DD catalytic mutant, they showed that editing is mediated by ADAR2DD, not PspCas13b...tested truncated ADARDDs to see if they could shrink the construct. They identified ADAR2DD(delta984-1090...mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA binding to decrease..., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing (Cox et al., 2017...