...Schematic of Opto-katanin’s use in cells. Images adapted from Meiring et al., 2022, under CC-BY 4.0 license...blue light illumination. Scale bar 25 µm. Images adapted from Mahlandt et al., 2023, under CC-BY 4.0 license...vesicle trafficking via CRY2-CIB1 clustering. Image adapted from Aljiboury et al., 2023, under CC-BY 4.0 license...colocalizing with SuperPLDx30. Scale bar 10 µm. Images adapted from Tei et al. 2022, bioRxiv, under a CC-BY-NC-ND...doi.org/10.1371/journal.pgen.1010765.  Berlew, E. E., Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C....

... on human artificial chromosomes. Montreal, QC, Canada 45.5017°N 73.5673°W – Vanessa Sung Hundreds of...de-funding of crucial environmental research programs, Canada has been here before.  These attacks on science...Research Centre at McGill University in Montreal, Canada.  Outside the lab, she dabbles in science policy...Intellectual Ventures Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – Karissa Milbury I attended...University of British Columbia in Vancouver, BC (Canada). She works in a lab that uses yeast cells to study...

...protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com...New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a...doi.org/10.1038/nn.4593 Chen, X., Ravindra Kumar, S., Adams, C. D., Yang, D., Wang, T., Wolfe, D. A., Arokiaraj...Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg,...Konno, A., Mochizuki, R., Shinohara, Y., Nitta, K., Okada, Y., & Hirai, H. (2018). Intravenous administration...Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw...Widrick, J. J., Messemer, K. A., Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz...

...product, or chromosome. One of the main causes of degradation during plasmid DNA extraction and purification...protein endonuclease I encoded by the gene endA. Nowadays, the most common E. coli strains used for plasmid... extra steps like heat inactivation, protein degradation, and/or the use of chelating agents that can ...store your sample without having substantial degradation and which experiments you can use your plasmid...

...SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal et al., Figure 1   Increased...curves for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. .../F0 for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. ... PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal...

...for the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered...and new tools for conditional expression and degradation. These workshops not only highlighted new tools...  Zhang, Liangyu, et al. "The auxin-inducible degradation (AID) system enables versatile conditional protein...

...purification of a specific genomic region, numerous adaptations of this system have been made in different fields...specific genomic region has also recently been adapted to isolate genomic loci in bacteria (Fujita et ...also has great potential for improvements and adaptations that can provide an increased understanding of...programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096 (2012): 816...

...endocytosed material or molecules targeted for degradation. These two pathways also use different cytoskeletal...several parallel mechanisms in place (for review see Maday et al., 2014). It is currently unclear which proteins...Common AAV Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes...PMID: 22196330. PubMed Central PMCID: PMC3275419. Maday, S., Twelvetrees, A.E., Moughamian, A.J., and Holzbaur...PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda...

...immune system. The techniques used here could be adapted to studying other systems in which there is large... revealing the cellular behaviors that mediate adaptive and innate immunity in diverse tissue environments... to better understand T cell initiation in the adaptive immune response. It was known that T cells were...scale when using photoactivatable FPs - their degradation within labelled cells limits the timespan available...

...developed by Roger Tsien and co-workers (Griffin, Adams, & Tsien, 1998), these dyes are now commercially...loops are best since these tags form beta-hairpins (Madani et al., 2009). Donor and acceptors: Putting it ...PMID: 19823671. 5. Griffin, B. Albert, Stephen R. Adams, and Roger Y. Tsien. "Specific covalent labeling...12125. Journal of the American Chemical Society. 7. Madani, Fatemeh, et al. "Hairpin structure of a biarsenical...

... On the 10th day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th...9th day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects🎵 On the 1st ...

...systems to generate ABE8e(TadA-8e V106W), which edits ~590-fold faster than the TadA from ABE7.10 without ...evolution to create one from the RNA adenine deaminase TadA.   After seven rounds of molecular evolution, they...editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science 353:aaf8729–aaf8729 . ...

...Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA domain is fused...editing system. Editors based on a second ADAR variant, ADAR2(E488Q/T375G), display improved specificity...alindromic R epeat (CRISPR) systems, which form an adaptive immune system in bacteria, have been modified ...target. Due to its comparative simplicity and adaptability, CRISPR has rapidly become the most popular ...hyperactive adenosine deaminase that acts on RNA, ADAR2(E488Q), to catalytically dead Cas13b creates a programmable...specificity, and editors carrying the delta-984-1090 ADAR truncation retain RNA editing capabilities and are small...recruit activation proteins. CRISPRi has also been adapted for diverse bacterial species using a modular system...

...AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically impaired Streptococcus pyogenes...Cas13 (dCas13) with the adenine deaminase domain of ADAR2. Now, they’ve further developed this editor for ...editing by evolving the adenine deaminase domain of ADAR2 into a cytidine deaminase. This editor still retains...

...Screenshot showing reagent entries with associated metadata adapted from the Affinity Reagent Working Group of...Human Biomolecular Atlas Program (HuBMAP) antibody metadata standards. And the dataset is rich. Experimental...

... particular screens of interest. Importantly, metadata for each screen is captured by BioGRID curators...gene hits for each screen are also captured as metadata.   Screen results are reported as the original... diversify, the BioGRID ORCS resource will be adaptable to a wide variety of screen formats and contexts...

...nationally and internationally COVID-19 Resources Canada is a database that provides information about reagents...research into COVID-19. While this program is based in Canada, much of the information is applicable to researchers...University of Toronto, co-founded the COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian...

...originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening viruses...scientists to hypothesize that prokaryotes developed an adaptive immune system that utilized various cas genes ... of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune system seems like the winning ticket to...

...University of Wellington, Victoria, has developed an adaptable bacterial expression vector which gives close ...and C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter...activation plasmids have great potential and have been adapted for use in mammalian cells, yeast, and Drosophila...during their transport. Finally, the system can be adapted to cellular screening to identify molecules that... overlap with the optogenetic actuator. In 2012, Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as...a tool for monitoring multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming...

...including organelles, are targeted to lysosomes for degradation. Autophagy is a dynamic process which involves...materials to be degraded to the lysosome, and degradation of autophagic substrates inside the lysosome....autophagasomal membranes, which leads to its degradation by autophagy. The RFP-LC3∆G, however, can not...