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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12 results for: amn

Showing: 1 - 12 of 12 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... step, they tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control...itself (leading to counter-selection escape). The rhamnose induction system is well-characterized and quite.... We placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal et al. also...demonstrated that the arabinose promoter worked too. Rhamnose induction relies on the transcriptional activator...manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS with a strong...protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...These vectors contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. ... not expressed as it is under the control of a rhamnose-inducible promoter. The resolution step leads ...and excision of the vector backbone. To do this, rhamnose is added which induces expression of the toxin...

  3. Bacterial Expression Systems

    Type
    Collection
    ...Escherichia coli Jay Keasling 113634 pSCrhaB2 PrhaB Rhamnose Burkholderia cenocepacia Miguel Valvano 47640 ... Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia sp. Silvia Cardona 44565 pST-KNarK2...

  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and Plant Parts Kit III (Gantner et al., 2018; Chamness et al., 2023). The Plant Parts II kit has a focus...for both monocots and dicots (Hahn et al., 2020; Chamness et al., 2023; Stuttmann et al., 2021; Grützner... https://doi.org/10.1371/journal.pone.0197185. Chamness, James C., et al. “An Extensible Vector Toolkit...

  7. dTAG - You're it!

    Type
    Blog Post
    ... the dTAG technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for...
Showing: 1 - 12 of 12 results