Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 11 results for: amn

Showing: 1 - 11 of 11 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... step, they tested their expression under both rhamnose- and arabinose-inducible promoters. Tight control...itself (leading to counter-selection escape). The rhamnose induction system is well-characterized and quite.... We placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal et al. also...demonstrated that the arabinose promoter worked too. Rhamnose induction relies on the transcriptional activator...manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS with a strong...protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...These vectors contain a toxin gene (regulated by a rhamnose-inducible promoter) and a chromoprotein gene. ... not expressed as it is under the control of a rhamnose-inducible promoter. The resolution step leads ...and excision of the vector backbone. To do this, rhamnose is added which induces expression of the toxin...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...and Plant Parts Kit III (Gantner et al., 2018; Chamness et al., 2023). The Plant Parts II kit has a focus...for both monocots and dicots (Hahn et al., 2020; Chamness et al., 2023; Stuttmann et al., 2021; Grützner... https://doi.org/10.1371/journal.pone.0197185. Chamness, James C., et al. “An Extensible Vector Toolkit...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Basics and Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...humans. Expression of this system is tunable via a rhamnose-inducible promoter. While this system was optimized...

  6. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... the dTAG technology. Follow him on twitter @behnamnabet.   References Nabet et al. The dTAG system for...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...books.google.at/books/about/Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...through "self-destruction" after induction with L-rhamnose and aTet. CRMAGE is much more efficient than traditional...
Showing: 1 - 11 of 11 results