... two global stock centers for Arabidopsis thaliana (Arabidopsis). Our mission is to collect, preserve,...in 60 countries each year. Introducing Arabidopsis Arabidopsis is an important model system for research...Translating Research on Arabidopsis Into a Network of Educational Resources). Arabidopsis is used in research... the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for...Ohio State University. Established in 1991, the Arabidopsis Biological Resource Center (ABRC) is one of two...distribute seeds, DNA and other resources for Arabidopsis and related species. Located at The Ohio State...globe. As a member of the Brassicaceae family, Arabidopsis is related to a variety of food crops such as...

..., a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons... are many motivations to generate transgenic Arabidopsis, from studying transcriptional and translational... plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation...transformation protocol, as well as protocols for growing Arabidopsis are also available (Weigel and Glazebrook, 2002...vector is the first step in generating transgenic Arabidopsis. There are a bunch of great options, but here... pool you will be selecting from later. The Arabidopsis transformation process The process itself is ... the chances of tDNA integration. Selecting Arabidopsis transformants Once your seeds from your T0 plants...

...angiogenesis inhibitor 1 Bax BCL2-associated X protein Bid BH3 interacting domain death agonist CASP3 Caspase...

...is as a biomedical model organism.  Arabidopsis thaliana Arabidopsis thaliana is one of, if not the most...community.  The Arabidopsis genome project marked the beginning of widespread Arabidopsis genetics research...characterized genome, and widespread utilization, Arabidopsis has made significant contributions to understanding...understanding plant and crop science alike.  Arabidopsis has many of the traits that make an ideal model organism...maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms is its...research development. Arabidopsis thaliana was fully sequenced by the year 2000, becoming the first plant...’s function. Some key genetics research with Arabidopsis include understanding of allelic variation between...

...occurs in T0 plants (from callus) or T1 plants (Arabidopsis floral dip). In the next generation the CRISPR...planning to use it to simplify my work with an Arabidopsis mutant that can’t set seeds. I study the development...   Fig. 3: (Top right) A RUBY-expressing Arabidopsis seed following floral dip transformation next...without antibiotics. (Top left) Mature silique (Arabidopsis fruit) with fertilized developing seeds from ... the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: CRISPR-Mediated Plant...

...   C. roseus is not a model organism such as Arabidopsis or tobacco. Studying gene function in C. roseus...method for overexpressing genes In plants like Arabidopsis and tobacco, Agrobacterium infiltration into ...expression assay by agroinfiltration of leaves." Arabidopsis Protocols. Humana Press, 2006. 225-229. PubMed...transient gene expression analysis in seedlings of Arabidopsis and other plant species." Plant methods 5.1 (...of our plant biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts...

...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (...tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of...in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more...Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with...designed for gene silencing in plants: microRNAs in Arabidopsis - Detlef Weigel. microRNAs in rice - Detlef Weigel... Weigel. microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system...Tools for an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens...

...functions in multiple species, including tobacco and Arabidopsis. Improving Agrobacterium binary vectors Vectors...domains and characterized in studies of tobacco, Arabidopsis, and corn. For both β-glucuronidase (GUS) and...efficiently inherited in both transgenic tobacco and Arabidopsis. Finally, Sahoo et al. demonstrate that this ...

... specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that the ...induced responses in Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she...salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression patterns in plants with enhanced...

...different mechanism thought to be associated with bidirectional transcription. In an uninfected cell, dsRNA ...interspersed nuclear elements can generate dsRNA by bidirectional transcription or as inverted repeats. These ...genomes which can produce dsRNA as a result of bidirectional transcription of their circularized DNA. These...

...off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow...inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis, C. elegans , sea squirt, cavefish, Chinese hamster...zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish...zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis, rice, sorghum, silkworm, and budding and fission...off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, rabbit...off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans , mouse, human, rat, yeast...

...tissue. 46968 pICSL01009::AtU6p : Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al....2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter. 46966 pICH86966::AtU6p::sgRNA_PDS...the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter. 48075 pICH86966 : A binary vector...

...expression patterns with reprogramming outcomes (Biddy et al., 2018). Tracking lineage and cell identity...tool. Check out the CellTag webpage! References Biddy, Brent A., et al. "Single-cell mapping of lineage...

...about Synthetic Biology Find Arabidopsis educational resources at the Arabidopsis Biological Resource Center...

...fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they call the LADL Anchor. Using... iterations is the addition of CRY2, another Arabidoposis protein that heterodimerizes with CIBN upon ...

...requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate particular...

...multi-cloning site flanked by T7 promoters to allow for bidirectional transcription to create dsRNA. Students can ...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...

... (MCS with a β-globin poly A) & EGFP from a bidirectional tet-responsive promoter (Pbi); Pbi contains ...Expression of your gene of interest & GFP from a bidirectional tet-responsive promoter (Pbi); Pbi contains ...Sabatini 92099 AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression of gene of interest...

...CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional, Cre-dependent...Dopamine Optogenetics Activators Inhibitors Bidirectional DREADDs Activators Inhibitors Other Molecular...

...amiRNAs based upon miRNA precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA...plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018...