We narrowed to 10 results for: cas9 2a egfp plasmid

...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...Cre recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed ...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination... expression. This plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and an sgRNA...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... measure time. These plasmids with different promoters driving expression of Cas9 were used in this study...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a ...targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...

...Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in a variety of ways. Cas9 proteins...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource... 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...backbone. Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused...Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425...

...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor...Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA... deposited the PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus... and the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous...sites. The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein... This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA.... Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology arms...

...out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression ...editing. How to use Addgene plasmids for Easi-CRISPR You can use Addgene plasmids to create a repair template...of Addgene plasmid options for templates - see the example at right using blue flame plasmid pmScarlet_...in Figure 2.  You can also use Addgene plasmids to purify Cas9 protein. The table below contains some ...desired insert (e.g., mScarlet).   Plasmid ID Plasmid Name Tags 69090 pMJ915 MBP (N ... 47327 pET-28b-Cas9-His 6xHis (C terminal); NLS (C terminal) 62933 pET-Cas9-NLS-6xHis 6xHis...pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology arms and...

...Engineering Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing...synaptophysin-EGFP for labeling of axon terminals. 1 Hongkui Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a. 5, rg*...AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA CMV-driven, constitutive Expression of human codon-optimized Cas9 from Staphylococcus...options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need.... mapping. 5 Jinhyun Kim 34912 paavCAG-post-mGRASP-2A-dTomato CAG-driven, constitutive Expression of post-synaptic...mutation. 1 Tsuneya Ikezu 203840 pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH hSyn-driven, constitutive hSyn-driven...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery and the...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs...

... Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human... MYL7-mEGFP AICSDP-46 mEGFP MLC-2a Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...

...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or...tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf 167935 pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP...Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids Blog: Inducible Promoters... for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi. See Plasmid #167937 for ...

...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1 SOD1 H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1...14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP...Dantuma 23971 VCP(wt)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23972 VCP(R155H)-EGFP VCP His, GFP, HA CMV...Dantuma 23973 VCP(A232E)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23974 VCP(DK0)-EGFP VCP His, GFP, HA CMV...Merrifield 28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP...Zuoshang Xu 28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP...