...determination (CFD score) is used to measure specificity of a guide to the target. The CFD score ranges ...performed because weak gRNAs (CFD < 20) bind almost not at all, and strong gRNAs (CFD > 80) bind almost as much...much as perfect gRNAs (CFD = 100). CFD scores (which range between 0 and 100) are then converted to a ... best practices. For example, it ranks guides by CFD score, and it mutates HDR donor templates to avoid... formula that does just this: Rank = (compressed CFD score) * (gaussian normalized cut-to-insert distance...mismatches between the guide and the DNA target. CFD scores are then modified (“transformed”) such that... PAM) in the target region after HDR. If the max CFD score is less than 0.03, it stops mutating. (This...

...U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific multiplex ...homepage. gRNA plasmids ID Name Description PI 49408 pCFD1-dU6:1gRNA expression of gRNA under control of the...the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression of gRNA under control of the Drosophila...Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression of gRNA under control of the Drosophila...Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs expression of two gRNAs from Drosophila U6...

...pyogenes Ward pCFD1-dU6:1gRNA 49408 Fly BbsI none S. pyogenes Virmilion Bullock and Port pCFD2-dU6:2gRNA ...DR274 42250 C. elegans BsaI none S. pyogenes Joung pCFD3-dU6:3gRNA 49410 Drosophila BbsI none S. pyogenes... Drosophila BspQI yes, cut S. pyogenes Puro Liu pCFD4-U6:1_U6:3tandemgRNAs 49411 Drosophila BbsI none ... U6 yes, cut (enhanced) S. pyogenes Puro Abbosh pCFD3-MS2tail-Scaffold 78906 Fly dU6:3 none S. pyogenes...zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for...from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery...

...Depositing lab Cas9 species = S. pyogenes (PAM = NGG) pCFD1-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection...

...Yiping Qi pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon pCFD5-NS Drosophila...

...growth factor receptor 2 BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25 FGFR3...