...U6:1 and U6:3 Bullock 73914 pCFD5 multiplex gRNA plasmid Bullock 73915 pCFD6 tissue-specific multiplex ...homepage. gRNA plasmids ID Name Description PI 49408 pCFD1-dU6:1gRNA expression of gRNA under control of the...the Drosophila U6:1 promoter Bullock 49409 pCFD2-dU6:2gRNA expression of gRNA under control of the Drosophila...Drosophila U6:2 promoter Bullock 49410 pCFD3-dU6:3gRNA expression of gRNA under control of the Drosophila...Drosophila U6:3 promoter Bullock 49411 pCFD4-U6:1_U6:3tandemgRNAs expression of two gRNAs from Drosophila U6...

...pyogenes Ward pCFD1-dU6:1gRNA 49408 Fly BbsI none S. pyogenes Virmilion Bullock and Port pCFD2-dU6:2gRNA ...DR274 42250 C. elegans BsaI none S. pyogenes Joung pCFD3-dU6:3gRNA 49410 Drosophila BbsI none S. pyogenes... Drosophila BspQI yes, cut S. pyogenes Puro Liu pCFD4-U6:1_U6:3tandemgRNAs 49411 Drosophila BbsI none ... U6 yes, cut (enhanced) S. pyogenes Puro Abbosh pCFD3-MS2tail-Scaffold 78906 Fly dU6:3 none S. pyogenes...zebrafish U6 promoters for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for...from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery...

...Depositing lab Cas9 species = S. pyogenes (PAM = NGG) pCFD1-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD3-dU6:3gRNA dU6:3 BbsI Injection or in vitro transcription...Virmilion none, need Cas9 plasmid Bullock and Port pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection...

...Yiping Qi pCFD3-NS Drosophila Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon pCFD5-NS Drosophila...

...growth factor receptor 2 BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25 FGFR3...