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We narrowed to 10 results for: cmv/1000

Showing: 1 - 10 of 10 results
  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression...hybrid mammalian promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit...
  2. Lentiviral Prep Service

    Type
    Collection
    ...17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter,...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9...
  3. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX...Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley JL. Use of the Gateway System for...
  4. Adenovirus Plasmids

    Type
    Collection
    ...Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter Vogelstein... pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene under CMV promoter ...pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957 RedTrackCMV Shuttle...mRFP-trackable viruses containing transgene under CMV Bamburg 50958 ShuttleNSE Shuttle For production of...mouse cofilin promoter (MCP) Bamburg 62621 pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with...in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular...
  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... DCTN1 CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina...hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1 SLC1A3 CMV Episodic ataxia ...moPrP(3F4) D177N PRNP CMV Dementia Susan Lindquist 1321 cytosolic moPrP (3F4) PRNP CMV Dementia Susan Lindquist...FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS Peter Howley 10880...DNMT1 GFP CMV Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 DNMT1 GFP CMV Hereditary...PINK1 Myc CMV Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson...C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark...
  6. Control AAV Preps

    Type
    Collection
    ...105531 pAAV.CMV.LacZ.bGH CMV LacZ Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase...Constitutive PHPeB Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Constitutive 1, 2, 5, 8, 9, rh10... 5, 8, rg*, PHP.eB Wilson 105548 pENN.AAV.CMVs.TurboRFP.WPRE.RBG CMV TurboRFP Constitutive 1, 8 Wilson...CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other... Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive...Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH Syn EGFP Cre dependent...
  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA...produces point mutations at a rate of up to 1 per 500-1000 bp. In comparison, the DNA replication error rate...their open access article. Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout...
  8. Promoters

    Type
    Guide
    ...interest is expressed. Summary Promoters are about 100-1000 base pairs long and are adjacent and typically upstream...Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from human...
  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity is... tdTomato 554 581 95 4.7 1 hr Tandem-dimer pCSCMV:tdTomato - Mammalian Expression pFA6a-link-tdTomato-...
  10. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...AAV1 pAAV.CMV.LacZ.bGH Control James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control...AAV5 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-5-PV0102 105531-AAV5 pAAV.CMV.LacZ.bGH Control...AAV8 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-8-PV0102 105531-AAV8 pAAV.CMV.LacZ.bGH Control...AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-9-PV0102 105531-AAV9 pAAV.CMV.LacZ.bGH Control...36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P 100037-AAV1 ...AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5 pENN.AAV.CMVs.Pl.Cre.rBG James...AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9 pAAV.CMV.Luc.IRES.EGFP.SV40 James...
Showing: 1 - 10 of 10 results