...expressed until the base editor corrects early stop codons within T7 RNAP. A phage encoding the deaminase ...the ABE8e plasmids at Addgene! Why engineer and optimize base editors? Targeted evolution of naturally ...

...CRISPR Blog Posts A CRISPR/Cas system designed and optimized for work in the model plant Nicotiana benthamiana...Plasmid 46965 pK7WGF2::hCas9 : Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823...

...arrestin recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression...provided in both open (no stop codon) and closed (including a stop codon) formats. The collection is compatible...directly for drug screening or easily shuttle the optimized, expression validated GPCR sequences to any desired...strains remains seriously limited, as prior optimizations are required for each individual target species...being generated, there is a need for genetic optimization of industrial yeast strains. However, industrial... the well-known yeast two-hybrid assay are not optimal for studying native mammalian protein interactions...transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila and yeast...

...less efficient than Cre recombinase. FLPe codon optimization resulted in FLPO, which shows similar efficiency...loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through smart engineering Different FLP...first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient in mammalian...identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart 1998)....

... Plasmids This plasmid collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible...

...technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and ...issues as traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require... emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution is to couple ...

... of a catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell ...

...residue peptide to the C-terminus of human codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana...

...delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease...

...amplified for cloning into one of three Zeamays codon-optimized Cas9-containing binary vectors. The system ...follow these same assembly principles, but they’re optimized for different purposes.   Gersbach Lab multiplexing...destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids are available for both monocot and... paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for efficient germline and somatic...Ronda, Carlotta, et al. (2016). "CRMAGE: CRISPR Optimized MAGE Recombineering." Sci Rep. 6:19452. PubMed...

...manipulations start. Anthony Forster’s lab used codon optimization programs to engineer 14 chromoprotein sequences...

...infectious particle production, including codon optimization, deletion of the C-terminal 18-21 amino acids...

..., S-tag, TAP tag, TRX, or V5) Wild type vs codon-optimized expression (e.g. wt or recode) ID Plasmid Description...

...and mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into ...

...20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

...pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about ...

...N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787...C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization...His-tagged cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent...His-tagged yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent ...

...guide that directs cleavage right before the stop codon!   Design your repair template with 55-100 base ...template with 55-100 bp homology on each side. For optimal repair, place the loxP sites precisely at the cut...

...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...