We narrowed to 105 results for: cre dependent fluorescent reporter

...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...Edward Boyden Voltage Fluorescent voltage reporter Voltron (Constitutive or Cre-dependent) Bright and photostable... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal ...photoconvertible fluorescent protein-based calcium integrator (CaMPARI) (Constitutive or Cre-dependent) Neural ... AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...

...find other Cre-, Flp-, or Dre-dependent plasmids. Return to top Recombinase-Dependent Reporter Plasmids ...luciferase and Cre to interrogate tumorigenesis in vivo using a mouse line expressing Cre-dependent Cas9. Kits...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre ... Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific...expressing Cre , Flp , Dre , other recombinases , as well as useful empty backbones or reporters . Experimental... interest. Using Cre or Flp as examples, here are a few popular recombinase-dependent gene expression ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...

...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter...AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP Viviana Gradinaru...AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

...Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060...regulatory element or gene of interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...

...and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent biosensors... of fluorescently-labeled components Fluorescence-activated cell sorting (for cells); fluorescent HPLC...Robert Campbell’s lab display calcium-dependent increases in fluorescence of up to 11,000-fold. FRET biosensors...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent protein is most...you use to detect your fluorescent protein? The type of detection method depends on what type of information...cameleon” calcium sensors created by Roger Tsien’s lab contain two fluorescent proteins (a FRET pair) separated...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...dTomato Cre dependent 1 Jonathan Ting 105714 pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Rylan... none Cre dependent 1 Kaspar Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent...none Cre dependent 1 GENIE Project 234442 pAAV-syn-flex-iGluSnFR4f-NGR-WPRE Syn iGluSnFr4f none Cre dependent...Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward...KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST...ST-WPRE Syn Voltron2-ST none Cre dependent 1 GENIE Project Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI...

...dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives...Addgene Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts...Although Cre isn’t a modular protein, Tang et. al were able to create a split version of Cre that is only...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP ...

...Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase...Multiplex: FRET reporters are comprised of two fluorescent proteins with very specific spectral properties...together. Challenging  to Design: FRET reporter activity depends on both the distance and the orientation...kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest which is...translocation of the reporter. As a result, the KTR is shuttled in and out of the nucleus depending on its phosphorylation...flexible swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited...they are easy to create and simple to multiplex. Problems with FRET kinase reporters Difficult to Multiplex...

... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence... element or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids...pC0037 Gaussia, Cypridina EF1α, CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina...imaging or creating biosensors. Read more about BRET on our blog . Nano-Lanterns: Fluorescent proteins ...Region) : Genome-wide screening of enhancers using a firefly luciferase reporter. These plasmids improve...

...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted syn-FLEX-axon-jYCaMP1s...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that exhibits...this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, ...in this post: Yeast-based protein evolution Fluorescent tools for mammalian cells Nanobody purification...enables tracking of protease activity with a fluorescent protein--no affinity purification required! The...doi.org/10.1038/s41592-019-0665-7 Optimizing fluorescent tools for labeling structures and compartments...mammalian cells By Angela Abitua Genetically encoded fluorescent proteins are often used to label  specific structures...

...and fluoresce. FlipCherry: Red fluorescence protease reporter To add to the fluorogenic reporter toolkit...genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been developed...results in a change in fluorescence emission. FRET and fluorescence based reporters however have several...name FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019)....be applied to other fluorescent proteins to create an array of multicolor reporters that will enable imaging...transfer (FRET)-based executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase...necessary for fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When...

...rate depends on power is a property of the fluorescent protein. This can differ between fluorescent proteins...desirable to have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may... the ideal situation, a fluorescent proteis should emit a stable fluorescence signal, showing no or little...This non-linear dependence on excitation power is important since different fluorescence imaging strategies...measurements for different fluorescent proteins and by comparing how the fluorescence intensity changes over...cyan fluorescent proteins is depicted in figure 1. Of note, the photobleaching rate does not depend on ...your understanding of reported data. Photobleaching results in a loss of fluorescence, which, in its simplest...

...background fluorescence and in-frame fluorescence.  Figure 2: The GEmCherry reporter. Image from...assays. Recently scientists have created CRISPR fluorescent reporter assays to quantify targeted Cas9 ...(NHEJ). In these reporters, editing typically results in a change in the fluorescent readout. In this ...this blog we will focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct...enrich for mutants through the presence of a fluorescent reporter. Lastly, once a mutant is identified, the...

... Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2...ratio Fluorescence (mCherry) Mycobacterium sp. Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase...

...  NIR reporters and biosensors NIR reporters can tell us about cellular events via  fluorescence changes...after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...proteins provide a scaffold for antigen-dependent fluorescent nanobodies. Nat Methods submitted (2021)...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers.... & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells...Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) were derived from natural ...

... and is endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically encoded... addition, ATLAS itself could be expressed Cre-dependently to allow tracing exclusively from genetically...in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses...these proteins are bright! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders...St-Pierre recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et ...small differences between mGold2s and mGold2t depending on the application. Figure 1: Super-...

...expressed independently) originally fluoresce green or blue and, when they mature, fluoresce red. The ...immature fluorescence from the FP timer is dependent on the total expression time but independent of protein...time.Hallmarks of fluorescent protein timers When FP timers are first expressed, they predominantly fluoresce one ...., et al. "Monomeric fluorescent timers that change color from blue to red report on cellular trafficking...Advice on Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse...Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve as a drug-screening platform...sequences which brings the attached fluorescent protein to the DNA. They created plasmids for purification of...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed...protein CZI, which in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression...

...successful screen, it is necessary to identify the gRNAs that elicit the phenotype, and this depends on the...whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ...luciferase downstream of the apoB gene. Using this reporter, the researchers explore many aspects of ApoB-...2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation in bacteria...However, the promoter in question must be σ54-dependent and the system may not be adaptable to distantly... systems and further the study of natural σ54-dependent promoter systems. Find these CRISPR activation...

...which have become common genetic reporters and alternatives to fluorescent proteins. Figure 1: Firefly...Properties of luminescence versus fluorescence   Luminescence Fluorescence Source of light Chemical reaction...brings the fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein emits...luciferase, releasing light. Created with BioRender.com. Bioluminescent reporters require a chemical substrate...the title of this post, this reaction is not fluorescence! Chemiluminescence, which includes all bioluminescence...of light by a chemical reaction. In contrast, fluorescence occurs when a molecule is excited by a high-...to be provided and are generally dimmer than fluorescent proteins. On the other hand, they don’t require...