...Lin Voltage Fluorescent voltage reporters Archon 1 and Archon 2 (Constitutive or Cre-dependent) A robotic...Edward Boyden Voltage Fluorescent voltage reporter Voltron (Constitutive or Cre-dependent) Bright and photostable... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal ...photoconvertible fluorescent protein-based calcium integrator (CaMPARI) (Constitutive or Cre-dependent) Neural ...(Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP A fluorescent, reagentless biosensor... AMP) Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr...

...Rho-Cre is expressed in the retina). Fluorescent Cre: The fusion of Cre to a fluorescent reporter enables...-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (...recombinant Cre Bacterial Rajewsky 13775 pCAG-Cre Cre-Myc CAG Mammalian Cepko 13776 pCAG-Cre:GFP Cre-GFP fusion...pOG231 NLS-Cre CMV Mammalian Wahl 19131 pIC-Cre Cre Bacterial Rajewsky 22776 MSCV CreERT2 puro Cre-ERT2 - ...mCherry-P2A-Cre Cre CMV RABV Jessell 73474 RabV CVS-N2c(deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft...comparison of Cre+ and Cre- cells. While Cre-lox recombination is a very useful tool, Cre expression can...pPGK-Cre-bpA Cre PGK Mammalian Rajewsky 11916 pBS185 CMV-Cre Cre hCMV Mammalian Sauer 11918 pBS513 EF1alpha-cre Cre...

...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. ...-EYFP An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter...AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP Viviana Gradinaru...AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

...and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent biosensors... of fluorescently-labeled components Fluorescence-activated cell sorting (for cells); fluorescent HPLC...Robert Campbell’s lab display calcium-dependent increases in fluorescence of up to 11,000-fold. FRET biosensors...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...Picking your reporter Don’t forget about the other end of the biosensor: which fluorescent protein is most...you use to detect your fluorescent protein? The type of detection method depends on what type of information...cameleon” calcium sensors created by Roger Tsien’s lab contain two fluorescent proteins (a FRET pair) separated...

...dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives...Addgene Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts...Although Cre isn’t a modular protein, Tang et. al were able to create a split version of Cre that is only...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP ...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Cre dependent 1 Podgorski 135421 pAAV-syn-FLEX-axon-jYCaMP1s Syn jYCaMP1s none Cre dependent 1 Podgorski...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent...none Cre dependent 8 Deisseroth 137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth...

...Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded fluorescent kinase...Multiplex: FRET reporters are comprised of two fluorescent proteins with very specific spectral properties...together. Challenging  to Design: FRET reporter activity depends on both the distance and the orientation...kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest which is...translocation of the reporter. As a result, the KTR is shuttled in and out of the nucleus depending on its phosphorylation...flexible swapping of fluorescent proteins and multiplexing is simple when fluorescent proteins with limited...they are easy to create and simple to multiplex. Problems with FRET kinase reporters Difficult to Multiplex...

...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted syn-FLEX-axon-jYCaMP1s...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that exhibits...this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, ...in this post: Yeast-based protein evolution Fluorescent tools for mammalian cells Nanobody purification...enables tracking of protease activity with a fluorescent protein--no affinity purification required! The...doi.org/10.1038/s41592-019-0665-7 Optimizing fluorescent tools for labeling structures and compartments...mammalian cells By Angela Abitua Genetically encoded fluorescent proteins are often used to label  specific structures...

...and fluoresce. FlipCherry: Red fluorescence protease reporter To add to the fluorogenic reporter toolkit...genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been developed...results in a change in fluorescence emission. FRET and fluorescence based reporters however have several...name FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019)....be applied to other fluorescent proteins to create an array of multicolor reporters that will enable imaging...transfer (FRET)-based executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase...necessary for fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When...

...virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV...Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca 2+] i Increase in Down Syndrome Mice, Ts1Rhr . Do you...SARS-CoV-2 entry, like that of SARS-CoV and MERS CoV, depends on binding of the viral spike (S) protein to cellular...method termed Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad... termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information...(Link opens in a new window) CRISPR tools and reporters now available from Stanley Qi's lab. COVID-19 ...

...rate depends on power is a property of the fluorescent protein. This can differ between fluorescent proteins...desirable to have fluorescent proteins that are photostable. On top of photobleaching, fluorescent proteins may... the ideal situation, a fluorescent proteis should emit a stable fluorescence signal, showing no or little...This non-linear dependence on excitation power is important since different fluorescence imaging strategies...measurements for different fluorescent proteins and by comparing how the fluorescence intensity changes over...cyan fluorescent proteins is depicted in figure 1. Of note, the photobleaching rate does not depend on ...your understanding of reported data. Photobleaching results in a loss of fluorescence, which, in its simplest...

...bioluminescent reporter occurs slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein...Fluorecent Proteins in Nano Lanterns Reporter gene assays A reporter gene, such as luciferase, usually serves...photons, chemiluminescent reporters are generally less bright than fluorescent proteins, but have the advantage...luminescence versus fluorescence.  Table 1: Properties of Luminescence versus Fluorescence   Luminescence... is also the most commonly used bioluminescent reporter. This beetle emits a yellow-green light with a...several studies utilized luciferase as a genetic reporter in plant and mammalian cells. Luciferase assays...available to choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In...

...background fluorescence and in-frame fluorescence.  Figure 2: The GEmCherry reporter. Image from...assays. Recently scientists have created CRISPR fluorescent reporter assays to quantify targeted Cas9 ...(NHEJ). In these reporters, editing typically results in a change in the fluorescent readout. In this ...this blog we will focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct...enrich for mutants through the presence of a fluorescent reporter. Lastly, once a mutant is identified, the...

...  NIR reporters and biosensors NIR reporters can tell us about cellular events via  fluorescence changes...after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...proteins provide a scaffold for antigen-dependent fluorescent nanobodies. Nat Methods submitted (2021)...directed evolution approach applied to fluorescent voltage reporters. Nat Chem Biol 14, 352-360 (2018). Yu...sets of near-infrared fluorescent proteins (NIR FPs) and biosensors have been created recently. As developers.... & Verkhusha, V.V. How to Increase Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells...Addgene. Biliverdin chromophore Near-infrared fluorescent proteins (NIR FPs) were derived from natural ...

...expressed independently) originally fluoresce green or blue and, when they mature, fluoresce red. The ...immature fluorescence from the FP timer is dependent on the total expression time but independent of protein...time.Hallmarks of fluorescent protein timers When FP timers are first expressed, they predominantly fluoresce one ...., et al. "Monomeric fluorescent timers that change color from blue to red report on cellular trafficking...Advice on Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse...Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...tetrameric fluorescent proteins can be. Cytotoxicity, improper localization, and decreased functionality...

...stranded DNA bound by tTALE-FP with the fluorescent protein fluorescing green. Image from Shin et al., 2019...intercalating fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein...Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve as a drug-screening platform...sequences which brings the attached fluorescent protein to the DNA. They created plasmids for purification of...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed...protein CZI, which in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression...

...27. See More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders... S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian cells. Lathuiliere... (AIS) plasticity with a motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function...antibodies and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq...

...successful screen, it is necessary to identify the gRNAs that elicit the phenotype, and this depends on the...whole organism imaging. They created an endogenous ApoB-NanoLuc reporter by inserting the NanoLuc luciferase...Cell, 2019. PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article ...luciferase downstream of the apoB gene. Using this reporter, the researchers explore many aspects of ApoB-...2019. Pubmed PMID: 31366908 Adapting sigma54-dependent transcription for CRISPR activation in bacteria...However, the promoter in question must be σ54-dependent and the system may not be adaptable to distantly... systems and further the study of natural σ54-dependent promoter systems. Find these CRISPR activation...

.... Two days later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected... the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

...and its cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent protein...DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-...constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2...exhibits high contrast between peak fluorescence and resting fluorescence. It is useful for activity imaging...exhibits high contrast between peak fluorescence and resting fluorescence.  It is useful for activity imaging...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium sensors and ...the endoplasmic reticulum, but exhibits larger fluorescent responses to GABA. Find the iGABASnFR and its...