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Showing: 61 - 80 of 195 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...few steps. More recently, CRISPR technology has adapted Golden Gate cloning for inserting the appropriate...single gRNA-expressing plasmid, but it can also be adapted to express multiple gRNAs. Addgene has two Golden...

  5. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    .../Cas9 for gene editing has expanded since its adaptation for use in mammalian cells in 2012-2013. Researchers... gene (although it has been quite successfully adapted for use in screening experiments as well). Therefore...

  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  9. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...

  12. Finding Your Perfect Job After University

    Type
    Blog Post
    ... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...black is the fusion tag or labeling system. Image adapted from Lavis, 2016, under CC-BY. Common self-...comparison with standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license...

  16. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... you probably realize by now, MoClo is a very adaptable system with lots of tools and reagents already...acssynbio.3c00423. Pérez-González, Ana, et al. “Adaptation of the GoldenBraid Modular Cloning System and...

  17. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...protocols, visit https://clover.caltech.edu. Figure adapted from Challis et al. 2022. Image created with BioRender.com...New World Monkeys and rodents. Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a...

  19. New Coomassie Protocol Video

    Type
    Blog Post
    ...Antibodies."  Hot tip! This protocol can easily be adapted for other types of proteins.    If this video ...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ...protocol was developed for 293T cells but can be adapted to a variety of target cell lines and selection...
Showing: 61 - 80 of 195 results