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We narrowed to 39 results for: ena/1000

Showing: 1 - 20 of 39 results

  2. Antibodies 101: ChIP

    Type
    Blog Post
    ...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...two-photon imaging with newer lasers in the range of 1000–1080 nm. These industrial fiber lasers are becoming...lasers, with two-photon excitation at 1030 nm, enabling two-photon imaging without compromising on brightness...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

  7. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge for efficient...systems. Importantly, they allow transient delivery, enabling transgene-free genome editing (no transgene integration... used to simultaneously target multiple genes, enabling efficient multiplexed genome editing in a single... diverse plant and animal systems, potentially enabling more precise and accessible genome engineering...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates... the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed repair and genome...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600   Choosing a photoactivatable...irreversible and reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible...Martin, et al. "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...This kit is built for serious multiplexing and enables users to express up to 7 gRNAs. The kit contains...

  12. Antibody Guide

    Type
    Collection
    ...sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process may...identity to be easily confirmed via sequencing, and enables the variable region and constant regions of the...given antigen. A mature B cell can clone itself to enable higher production of a single antibody, but each...blot Western blots use antibodies to identify denatured proteins separated by size on an SDS-PAGE gel....additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals, and run...primary antibody must be able to recognize the denatured configuration of the protein. For western blots...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...produces point mutations at a rate of up to 1 per 500-1000 bp. In comparison, the DNA replication error rate...lentiviral CRISPR library     CRISPR pooled libraries enable researchers to screen the entire genome for genes...way scientists create new traits in bacteria and enabled the in-depth study of many biological processes...only genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing...temperature-controlled expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide...developed CRISPR tools for epigenome editing that enable the precise study of particular epigenetic modifications....1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 Boris Greber pTAL7a...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Hemocytometer or cell ...system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration Unit, Nalgene...solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes at -80 °C... deionized water and adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

  15. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...interest. If you are working with cells that are amenable to genetic knockout, this represents a very robust...essential to the cell’s function, it likely is not amenable to knockout approaches. In these cases, we would...synaptotagmin 1 page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach...be difficult to achieve sufficient knockdown to enable robust assessment of antibody selectivity. Additionally...). Scaling of an antibody validation procedure enables quantification of antibody performance in major...Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. ...

  16. AAV ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...calculate the titer for each sample: $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume...min) Ramp Rate (°C/sec) # Cycles Denaturation 95 10 2 1 Denaturation 95 0.5 2 50 Annealing/Extension 60...

  17. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Pipette controller Microcentrifuge...Pipette tips, 200 µL, VWR 76322-150 Pipette tips, 1000 µL, VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 ...and ImageJ software. The sample is separated by denaturing polyacrylamide gel electrophoresis alongside ...

  18. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... organization issues – F1000 just announced the launch of their F1000Workspace.  Give your favorite searching...where I dump all of my papers and they all have filenames (year first author name). It’s rudimentary, but...Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  19. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...integrate permanently into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral.... This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral Expression) system...

  20. Molecular Biology Reference

    Type
    Guide
    ...fluorescent signal. Sanger can sequence approximately 500-1000 bases downstream of the known primer region with...Recombinant DNA Technology Techniques in chemistry enable isolation and purification of cellular components...the field of recombinant DNA technology, which enables the preparation of more managable (i.e., smaller...are relatively small (∼3000 - 6000 basepairs) to enable easy manipulation. Typically plasmids contain the...molecular cloning . The process of molecular cloning enabled scientists to break chromosomes down to study their...which contains several restriction enzyme sites, enabling easy insertion of DNA by restriction enzymes digestion... purpose of replication. The selectable marker enables selection of a population of cells that have taken...
Showing: 1 - 20 of 39 results