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We narrowed to 5 results for: gfp-lc3

Showing: 1 - 5 of 5 results
  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified LC3, GFP-LC3 is conjugated...in red puncta. Find the mRFP-GFP-LC3 plasmid at Addgene! GFP-LC3-RFP-LC3∆G: A fluorescent probe that releases...autophagy biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein...chain 3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as...Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and GFP fused to the... a decrease in autophagic flux.   Find the GFP-LC3-RFP-LC3∆G plasmid at Addgene! References Klionsky,...Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence relative to RFP fluorescence...
  2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ATG8 is part of a group of genes that ...autophagosomes and autophagic bodies in yeast. A GFP-tagged version of LC3, the mammalian Atg8p homolog, can also...with autophagosomal membranes, so tagging it with GFP allows for tracking the localization or accumulation...autophagy-independent manner. Additionally, tracking Atg8p or LC3 doesn’t provide information about the contents of...in the vacuole or lysosome, since both Atg8p and LC3 are degraded or released once an autophagosome fuses...pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). They are ...Fluorescent Protein Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21217 ... EGFP Tamotsu Yoshimori 110060 FUW mCherry-GFP-LC3 Autophagosomes LC3 mCherry, EGFP Anne Brunet 61204 ...61803 GFP-Rab7A Endosomes (late) Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Endosomes (late) RAB7 GFP Richard...Protein PI 11546 EGFP-LC3 Autophagosomes LC3 EGFP Karla Kirkegaard 40827 mCherry-hLC3B-pcDNA3.1 Autophagosomes...pMXs-IP GFP-WIPI-1 Autophagosomes WIPI1 EGFP Noboru Mizushima 21074 ptfLC3 Autophagosomes LC3 mRFP1, EGFP...Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens...Autophagosomes LC3 mCherry David Rubinsztein 38277 pMXs-puro GFP-p62 Autophagosomes Sequestosome-1 EGFP Noboru...
  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an ...fluorescent-tagged LC3. Autophagy. 2007;3(5):452-60. Tamotsu Yoshimori Autophagy Expresses pHluorin-mKate2-hLC3 (PK-...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP...
  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein...101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin...87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20 Mitochondria...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin... SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin...101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin...
Showing: 1 - 5 of 5 results