...Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids 101 Posts...as this will be useful for the ligation step. 3. Ligate Your Insert into Your Vector In the ligation step...

...concentration of 1 μM). Ligate annealed oligos into pX330 using a Golden Gate assembly cloning strategy...

...allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin... of CRISPR technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi..., gene knockout strategies cannot be used to investigate essential genes. Historically, gene silencing... which allows easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection...from Worcester Polytechnic Institute where he investigated how plants achieve polarized cell growth in ...

...spacers/gRNAs are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized...

...engineering. These components are provided in Golden Gate compatible backbones that can be combined with...all the latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system...system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists...neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit...empty vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your...toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the...2007. PubMed PMID: 17937395 Listen to Our Tol2 Gateway-Compatible Toolbox Podcast Segment The impact of...

... John W. Campbell. This period is termed as the golden age of SciFi, but the common themes that these ...between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing...

...a microscopist, a biostatistician, and that one golden-handed technician who can do a certain difficult...someone to coordinate, someone to decide, someone to delegate, etc. If you find that there is not someone (or...