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We narrowed to 37 results for: halo tag

Showing: 1 - 20 of 37 results

  1. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...degron tags. What is a degron tag and why should I use one?   Degron tags are a class of protein tag which...amenable to being tagged, e.g. tagging some proteins can affect their function, the SMASh tag self-cleaves ...). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend a lot on...Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation tag (dTAG) system functions...degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and the endogenous cellular...machinery to function. The dTAG is the smallest tag reviewed here, in regard to total tag size, which can also...

  2. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene... et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National Academy of Sciences111.36... circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553....

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed... scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic...between the tag and homology arms). Digestion of the donor plasmids with SapI allows the tag, selectable... inserting a tag at multiple target sites. SapTrap vectors can be easily modified to tag hundreds of genes...insertion tagging), developed by the Hornung Lab, allows users to easily create C-terminal tag fusions ...endogenous protein tagging, please visit our website CRISPR/Cas Plasmids - Protein Tagging. Beyond simply...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Stamm 204222 CMV-Halo-10-PFN1 PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla 204223 CMV-Halo-10-PFN1-Y6D PFN1...PFN1 Halo, Tev CMV ALS Jessica Henty-Ridilla 204224 CMV-Halo-10-PFN1-R88E PFN1 Halo, Tev CMV ALS Jessica...229738 pPB-EF1A-HALO-hLRRK2 LRRK2 Halo EF1-alpha Parkinson's Shawn Ferguson 229742 pPB-EF1A-HALO-hLRRK2 (LIR1... 161767 FUS_Halo_N_allele_400 FUS Halo ALS Michael Ward 161768 FUS_Halo_C_allele_400 FUS Halo ALS Michael...Melcher 178115 ANG_Halo_C_allele ANG Halo ALS Michael Ward 178116 PRNP_Halo_C_allele PRNP Halo Dementia Michael...178117 PFN1_Halo_C_allele PFN1 Halo ALS Michael Ward 178118 CHCHD10_Halo_N_allele CHCHD10 Halo ALS Michael...178119 UBQLN2_Halo_C_allele UBQLN2 Halo ALS Michael Ward 178120 SIGMAR1_Halo_C_allele SIGMAR1 Halo ALS Michael...

  5. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575...genetic tag on the protein of interest. Table 1: Summary of popular self-labeling tag systems Tag Size...than short peptide tags like tetracysteine (Jing & Cornish, 2011). HaloTag and SNAP-tag are the most popular...application, SNAP-tag, CLIP-tag (both 182 aa), and eDHFR (159 aa) are smaller than HaloTag (295 aa) – for...flexibility of self-labeling protein tags. The basics These tags most often consist of an enzyme that ...resorufin). SNAP-tag: In 2003, Kai Johnsson’s lab developed a self-labeling fusion tag based on the human...later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The...

  6. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...self-labeling tags on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian...Expression pSNAP-tag (T7) Vector - Bacterial Expression CLIP-Tag Benzylcytosine (BC) pCLIP-tag (m) Vector -...Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo - Xenopus Expression... pET51b-His-TEV-HaloTag7 - Bacterial Expression SNAP-Tag Benzylguanine (BG) pSNAP-tag (m) Vector - Mammalian...Bacterial Expression Return to top Self-Labeling Tags These tags have been engineered to covalently bind a synthetic...empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest...Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources The following tables list many...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... of N- and C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for...electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared to traditional...promoter, N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and...of an isopeptide bond between 2 peptide tags, SpyTag and KTag. The group demonstrated the use of the SpyLigase...expression levels. APEX2 is a monomeric, 27 kD genetic tag which can be fused to proteins of interest for EM...same animal. Flipbow additionally incorporates SUMO tags in the FP sequence for separation from Cre-based...levels to be directly compared among the different tags To clone a sequence or gene of interest into the...

  8. Optogenetics Guide

    Type
    Guide
    ...enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR...strain Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...function. TULIPs Tunable light-inducible dimerization tags (TULIPs), plasmids allow cloning of protein coding...sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk Blue-light photoreceptor for light-...plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula (Halobacterium...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...TREAT : Biosensor using Renilla luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...Lentiviral expression of firefly luciferase with a V5 tag Kevin Janes 99283 pCW57.1-Luciferase Firefly TRE ...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (neo)...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...luciferase . Each type of luciferase has advantages and disadvantages depending on the application, and different...Constructs using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors...

  10. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...CLIP-tags Tetbow SNAP-tag, CLIP-tag, HaloTag Based on the Brainbow system, adds Tetracycline (Tet) transactivator...SNAP-tag, CLIP-tag Plasmids for visualization of proteins in live or fixed cells using SNAP-tags or CLIP-tags...VirD2 HUH-tag For creating DNA-protein conjugates rHUH HUH-tag (RNA) Covalent protein tag for RNA labeling...attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection HaloTag Collection...ligands to HaloTag PCV-Cas9, Cas9-PCV HUH-tag Cas9 plasmids with N- or C-terminal PCV2 HUH-tag; increase... and Let Dye: Self-Labeling Protein Tags Plasmids 101: Degron Tags Additional Resources on addgene.org...tube or in cells. There are also SNAP-tag and HaloTag modules, which can connect proteins to any kind ...

  11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...these self-labeling proteins are SNAP-tag® and HaloTag®. These tags consist of a genetically encoded enzyme...several advantages to using these self-labeling proteins over traditional fluorescent protein tags, such...endogenous target, and thus no genetically encoded tag is required. These dyes can also be attached to an...ligands have more flexibility due to the affinity tag they typically bind to. Stability of fluorescence...experimental labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression...fluorescent experiments, such as using fluorescently tagged ligands or intermolecular FRET for ligand-receptor...should possess high specificity to ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET...

  12. Hot Plasmids - February 2022

    Type
    Blog Post
    ... small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag can recruit multiple...SNAP-tag, and HaloTag fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. ...b) Schematic of anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of...blotting, and more. Given the popularity of FLAG and HA tags, you may not even need to clone new constructs to.... The proteins, which are purified via histidine tags, are optimized for SDS-PAGE gels and can be detected...editing within a single expression cassette is advantageous to Cas9’s larger expression requirements (Figure...

  13. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...experiment.  Highlighted Split-Fluorescent Protein Tags Browse the table below, and click the Article link...cellular proteins with split fluorescent protein tags. Tamura et al. Commun Biol. 2021 Daichi Kamiyama...most-requested GFP(1–10) and GFP(11) Versatile protein tagging in cells with split fluorescent protein. Kamiyama... monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. Shcherbakova... Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow or orange-red fluorogen...

  14. Genetic Code Expansion

    Type
    Collection
    ...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS C6 C6 HaloTyrosine...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Mammalian TAG Kensaku Sakamoto 197099 pIYN3 TyrRS M. jannaschii halogenated tyrosines Bacterial TAG Kensaku...nitrotyrosine (3-nitro-Y), 3-halotyrosine (3-halo-Y) Bacterial TAG Ryan Mehl 214359 pAJE3-E7 aminoacyl-tRNA...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Bacterial TAG Dieter S√∂ll 104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David...

  15. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...other fluorescent proteins and tags including mCherry, HaloTag and SNAP-tag for multi-color imaging. Find...observed colocalization of GFP tagged HA frankenbody with an mCherry, 4xHA tagged histone protein (H2B) in ...could also bind a 1xHA tagged histone protein as well as a mitochondrial protein tagged with spaghetti monster... the 10xHA tag increased the fluorescent signal to noise ratio five fold over the 1xHA tag.  Lastly, the...use GFP to image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein...limitations, scientists have co-opted antibodies for tagging purposes. Different antibodies bind different protein... called epitopes and scientists can use these to tag their proteins of interest. Antibody based probes...

  16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, TMP-tag) – These widely used systems consist...strategies have two parts: (1) a genetically encoded “tag” expressed as a fusion with your favorite protein...fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the ...genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins in cells (Figure ...consist of a genetically encoded enzyme variant tag that reacts specifically and irreversibly with a small... attachment of a fluorophore ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine...paclitaxel. Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    ...lentiviral vectors to stably or inducibly express tagged iPAK4 from different promoters, specific time points...plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record.... B) HEK cell with an iPAK4 fiber labeled with HaloTag-iPAK4. Scale bar 10 µm. C) Images of iPAK4 fibers...fibers in cultured rat hippocampal neurons. HaloTag dyes are switched from yellow to red at 0 h and back...

  18. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fact, NLuc is bright enough to detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally..., NLuc is very inviting for endogenous protein tagging as it is only 19 kDa. An example is the CRISPaint-NLuc...2016) CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nature Comm...membrane-inserted voltage sensing domain (LOTUS V; Luminescent Optical Tool for Universal Sensing of Voltage). With...information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase...available through Addgene (Plasmid #83926). Live-Cell Voltage Sensor  Ingaki and colleagues (8) were interested... al. (2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of...

  19. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...allow for noninvasive live imaging with fluorescent tags and activation of light sensing proteins. Furthermore...become a popular model organism because their larval stage lends itself well to studies of neuroscience. The..."Optical control of zebrafish behavior with halorhodopsin." Proceedings of the National Academy of Sciences...

  20. Promega Plasmid Collection

    Type
    Collection
    ...analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting...detecting and tracking proteins using tags such as NanoLuc, HaloTag, SmBiT, and LgBiT. Ordering & Availability...reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other terms...Fusions HiBiT is a small, multifunctional epitope tag enabling precise protein detection. It can be paired...protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link opens in a new window) technology...applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters...your hands, thanks to interchangeable ligands. HaloTags form a covalent bond with these synthetic chemical...
Showing: 1 - 20 of 37 results