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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4 results for: hepatitis -(IRES) -(crispr)

Showing: 1 - 4 of 4 results

  1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest...: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element...this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before,...

  2. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...Nanoblades to Existing CRISPR Delivery Methods When compared head-to-head with existing CRISPR delivery technologies...delivery Learn about CRISPR delivery methods for mammalian cells Browse our CRISPR 101 blog post Resources...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral...microinjecting zygotes with CRISPR components. However, this sometimes requires intracellular injections ...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability...ability to infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation...pluripotent stem cells (hiPSCs). Nanoblades, a new CRISPR delivery method developed by the Ricci Lab and ...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...just after the R sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element;...as a promoter on the transfer plasmid, and this requires Tat for activation. Second-generation plasmids...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...replication and packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element;...central (CNS) nervous systems. Targeting the CNS requires crossing the blood-brain barrier (BBB), and a ...
Showing: 1 - 4 of 4 results