...earning their PhDs (3), showing that the ‘standard’ pathway into academia may in fact be just another ‘alternative...recently launched a survey “Identifying Career Pathways for PhDs in Science”, through which she collects...

...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

... in different steps in the glycan biosynthetic pathway and have a wide range of clinical manifestations...lines to study how immune processes and biological pathways are altered in PMM2-CDG. These studies are expected...

...further, his lab is now studying how Argonaute pathways may be involved in preventing silencing of self-genes...self-genes. Dr. Mello presented a hypothesis that these pathways are involved in RNA based surveillance of repetitive...

... using the endogenous homology directed repair pathway, a new user-defined DNA sequence (like GFP) can...previously unknown protein functions. References Schwartz, Matthew L. and Eric M. Jorgensen. “SapTrap, a...

...associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein might...undergraduate research experiences (CUREs) as a pathway to diversify science. Evolution and Development...

...since essential and non-essential genes for both pathways had previously been identified (Hart et al, Bassik...systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility." Cell 152.4 (...

...different sites in various endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The expression... pMLM3613 ). Additional details can be found in Hwang & Fu et al., Nat Biotechnol. 2013 and users are ...

...different target sites in endogenous zebrafish genes (Hwang & Fu et al., Nat Biotechnol. 2013). The Cas9 expression...expression of Cas9 in zebrafish can be found in Hwang & Fu et al., Nat Biotechnol. 2013. The guide RNA...

...and more, often driven by Rho family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups for applications...

...repaired by the nonhomologous end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, ...homology-directed repair (HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously...

...genome editing. The homology-directed repair (HDR) pathway is downregulated in mature tissues - no problem... through the non-homologous end joining (NHEJ) pathway. It’s estimated that very little dystrophin correction...

...silencing pathways: transcriptional and post-transcriptional gene silencing (PTGS). In either pathway, short...

...egularly I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress...mutagenesis ( Cong et al. , Mali et al. , Jiang et al. , Hwang et al. ), is that the nuclease-deficient mutant ...

...body, or they can be used to activate cellular pathways by binding to cell surface receptors. These applications...are typically used for investigating signaling pathways or developing therapeutics. Experimentally, antibodies...

...approved monoclonal antibodies targeting the PD-L1 pathway, VEGF production, and CTLA4 and CD protein blocking...Research Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ...

...general repair pathways: The efficient but error-prone non-homologous end joining (NHEJ) pathway The less efficient...high-fidelity homology directed repair (HDR) pathway The NHEJ repair pathway is the most active repair mechanism...

...because the non-homologous end-joining (NHEJ) repair pathway is not very robust. In many cases, homology-directed...natural products. To easily explore and engineer pathways within this genus, Huimin Zhao’s lab created two...

...fibroblasts via microRNA-dependent and -independent pathways. EMBO Mol Med. 2017 Aug;9(8):1117-1131. Parmar...modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into...

... a microbial pathogen, they trigger signalling pathways that lead to the activation of an immune response...PIG4, SAA, TP53I4 SAA2 serum amyloid A2 - SBDS Shwachman-Bodian-Diamond syndrome CGI-97, FLJ10917, SDS,...of alpha-syn(A53T). Target Accelerator Genetic Pathway Reference Set : From Jesse Boehm , William Hahn...ORFs that represent over 25 cellular signaling pathways and processes. Addgene Blogs Adeno Associated ...