...popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging, check...the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution...journal.pone.0082009. (PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page,...

...origin of replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll.... Chicken version is commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian...development-specific promoters available to scientists. Plasmids are oftentimes put to therapeutic uses, and in...Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about...Luciferase and GFP Resources on Addgene.org Find Plasmids for Your Research     ...

...Additional Resources on the Addgene Blog Read other Plasmids 101 Posts Learn How to Use Blue White Screening...

...Collection More resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden...

...Over-Agar Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance...Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your...antibiotic. The use of antibiotic resistance DNA plasmids are traditionally grown in bacteria as a way to...produce more of the plasmid, isolate individual plasmids, or express a gene contained in a plasmid. During...your experiment — to grow, express, or isolate plasmids. The presence of an antibiotic resistance gene...bacterial and eukaryotic selection, whereas other plasmids must carry both a bacterial and a mammalian antibiotic...Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

...in the Lab Plasmids 101: Screens vs. Selections Plasmids 101: Knockout /Knock-In Plasmids ...the Lab Bench Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons...

... for More Plasmids 101 Articles?  Find them all here: http://blog.addgene.org/topic/plasmids-101   ...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed that...BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB... strains is not recommended as these auxillary plasmids may be isolated during the prepping process. How...expression work? As mentioned above, many expression plasmids utilize inducible promoters, which are 'inactive...replication, making it incompatible with other p15A plasmids. pLys comes in two flavors—pLysS and pLysE—the...

...Addgene blog Plasmids 101: What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...'ll make it easier for you to find and use the plasmids you need. Here's what you'll find in this article... Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited cell populations...populations without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that... CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...'ll make it easier for you to find and use the plasmids you need. Here’s what you’ll find in this article...new toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward...system, the approach is intended to be amenable to organoids derived from other tissue-types. By bringing together...available from Addgene, head over to our CRISPR Plasmids and Resources page. Here are some highlights from the...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...'ll make it easier for you to find and use the plasmids you need. Here's what you'll find in this post...head over to our CRISPR Plasmids and Resources page.  A new system uses light to deactivate photocleavable... accumulation of indels to measure time. These plasmids with different promoters driving expression of...containing vectors developed in the Jordane Dimidschsteind lab provide expression selectively in Parvalbumin...

... enter the cells so they can go to work. Plasmids, plasmids everywhere To find out more about gene edited...expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag a wide...wide variety of genes with GFP. These plasmids are available through Addgene, and the stem cell lines we...found in the Cell Catalog on our data portal. The plasmids should be functional in many different human cell...constructs below. Read on to find out how these plasmids work and to discover more resources for your genomic...tagging strategy   The first step in creating the plasmids was to identify the gene of interest and decide...this manner, we have created an initial set of 10 plasmids representing 10 human genes and will add more ...

...possible, we propagate plasmids in the standard cloning strain DH5α. For plasmids with highly repetitive...lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids containing the ccdB gene...username. In order for the plasmids to appear in your Addgene account under My Plasmids, we need to know your...quickest ways to submit deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid...Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control  In this post, we...to send liquid DNA or bacterial streaks of your plasmids back to us. Our scientists will contact you if...Figure 1). Please read our “How to Deposit Your Plasmids with Addgene” blog post for a thorough guide to...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...head over to our CRISPR Plasmids and Resources page.  Plasmids for delivering CRISPRi components by a single...'ll make it easier for you to find and use the plasmids you need. Here's what you'll find in this post...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic ... Haynes at Emory recently contributed a set of plasmids to the repository that will help epigenetic researchers...proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...'ll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a ...mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the viral service A fast blue-light...head over to our CRISPR Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair... two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered prime editors...the plasmid here Jesse Zalatan’s lab deposited plasmids for a chemically inducible CRISPRa system in yeast...

...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...synthesis Ptrc-ccdA   Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR ...for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency supE44...Survival 2 T1R   TOP10 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...Streptomycin  HB101 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...DH5alpha   General cloning and storage of common plasmids, blue/white screening. F- endA1 glnV44 thi-1 recA1...common lab strain for cloning and storage of pBR322 plasmids.  F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara...

...Heidelberg University. “What is different is the plasmids you use to bring this into your target organism...

...plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but others...kanamycin-containing agar. Only cells carrying plasmids that contain a kanamycin resistance cassette will... for cloning are auxotrophic for specific amino acids. When plated on media lacking the essential amino... created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: ... property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is ...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Learn more about Lambda Red recombineering...counter-selection Resources on Addgene.org Find plasmids at Addgene Read our molecular biology reference...

... Gulp! Though bacteria don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes...’s genome. To do this scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles...find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations at ...requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the other...resources on the Addgene blog Read all of our Plasmids 101 blog posts Check out all of our blog posts...

... of LIC is often limited to specially-designed plasmids. Sequence- and ligation-independent cloning: A...recombination that will be further detailed in an upcoming Plasmids 101 post. Instead of using T4 DNA polymerase, ...